Ester Kalef Ezra Email & Phone Number

London Area, United Kingdom

London, England, United Kingdom

• Project: Investigate the nature and roles of somatic CNVs in Parkinson's Disease
• Optimized and employed multiple methodologies for single-cell isolation, scWGA (single-cell whole genome amplification), library preparation for scWGS (single-cell whole genome sequencing), scRNA-seq and scDNA/RNA.
• Assisted in single-cell long read calls validation to test Sniffles2 tool
• Recognized for teaching and supervision activities as an Associate Fellow of the Higher Education Academy
• Volunteer: - Interview Panel member- UCL IoN (Institute of Neurology) Equality, Diversity & Inclusion committee member- UCL IoN PhD Cohort co-lead for Welcome Event planning- Aligning Science Across Parkinson’s (ASAP).

Uxbridge, London

• Marked >40 UG Life Science students on written essays and oral presentations (~200 hours).
• Led a laboratory practical for MSc students and adapted the procedures to Covid-19 restrictions (~10 hours).

Uxbridge, London, UK

• Investigated metabolic signature of Friedreich’s ataxia (FRDA) & studied FRDA mouse models:
• Authored a publication in Frontiers in Neuroscience related to my study characterizing the characteristics of novel humanized FRDA mouse models and co-authored in Front. Genet. epigenetic therapeutic approaches for FRDA.
• Examined the role of sphingolipids in FRDA using primary fibroblasts to perform metabolomics and lipidomics analysis from multiple donors. For some experimental needs, I handled more than 40 cell flasks.
• Coached and directly day-to-day supervised 4 students and 2 technicians/interns in the laboratory.
• Acted as point of contact in the laboratory for various activities, such as purchasing orders and deliveries.
• Lectured in an online MSc class for which I received very positive feedback from the students.

London, United Kingdom

Research: Optimized and performed cell migration assays and immunohistochemistry experiments to study the mechanism of Sulf1/2 function in cardiovascular development and myocardial repair.Leadership: Educated 2 MSc students in the lab on cell culture methodologies and data analysis.

Stockholm, Sweden

• Enabled the laboratory setup and studied the role of ER and mitochondrial in skin wound healing:
• Performed drug screening scratch assays on multiple human primary skin fibroblasts and Western Blot assays.
• Analyzed and presented the results in local scientific meetings.
• Assisted the team to successfully secure further funding by editing draft proposals and optimizing methods.
• Created and organized human donor biobanks (tissues and patients’ fibroblasts).
• Organized initial laboratory set-up from the beginning and purchased consumables and related equipment.

Newcastle Upon Tyne, United Kingdom

Project: Intra-mitochondrial gene expression. Analysis of the role of Mitochondrial Ribosomal Protein L41 (MRPL41) in the process of protein synthesis and integration of proteins into the inner mitochondrial membraneAchievements: Successfully generated 5 plasmid constructs & developed 4 CRISPR/Cas9 human KO MRPL1 cell lines.

Department Of Biology - University Of Crete

• Dissertation: Mitochondrial targeting of human GDHs: presequence structure, function and evolution Supervisor: Prof. Kostas TokatlidisAdvisors: Assoc. Prof. Despoina Alexandraki, Assist. Prof. Babis SpilianakisAssist..
• Generated 33 plasmid constructs by molecular cloning and 19 plasmids by site-directed mutagenesis. Performed multiple mitochondrial assays to assess how hGDHs are imported into mitochondria.
• Investigated the evolution of the mitochondrial targeting signal of hGDHs in silico, in organello, and in culture.
• Presented the results in numerous prestigious conferences and written research grant reports
• Publicized results as 1st joined author in Bioch. J. and co-authored 2 review studies (Biology, Neurochem. Res).Demonstrated laboratory practicals to UG students for 3 semesters (volunteer work).

Master'S Degree, Protein Biotechnology, Excellent

M.Sc. thesis (1-year long): Genetic and biochemical analysis of oxidative protein folding in the mitochondria. Thesis defended on.

Specialized Graduate Training Program, Laboratory And Medical Genetics, Excellent

Distant Specialized Graduate Program (03/10/2016 - 30/06/2017) Final Grade: Excellent Specialized Graduate Training Program (~280 h of.

Bachelor'S Degree, Biology, Very Good

Activities and Societies:• Courses: Cell Biology, Molecular Biology, Developmental Biology, Genetics, Microbiology, Biochemistry.

High School (Honour'S Degree), Excellent

Accredited Public Greek High School • Accredited Public Greek High School • Mandatory Courses: Biology, Physics, Chemistry, Mathematics.