Ethan Hoffman Email & Phone Number

Ingelheim Am Rhein, Rhineland-Palatinate, De

• Design, plan and execute biotherapeutic experiments • Perform antibody production, expression, purification, and characterization• Culture and maintain CHO cell lines• Perform molecular biology experiments like High Throughput Cloning using Infusion, Golden Gate Assembly, Gibson Assembly, PCR, DNA isolation• Expression and purification of proteins using High Throughput Processes (HTP) and methodologies• Provide training and guidance to laboratory personnel• Prepare technical reports, publications, and oral presentations• Leverage gene analysis tools like Geneious for DNA editing and mapping• Utilize automated platforms like Beckman Coulter BioMek i7 Automated Liquid Handler, Hamilton Microlab STAR Automated Liquid Handler, GeneScript AmMag Quatro Automated Plasmid Purifier, & Agilent AssayMAP Bravo Automated Liquid Handler• Utilize high throughput analytical equipment like Nanotemper Prometheus Panta, Agilent aSEC, Sartorius Octet, QPix Microbial Colony Picker, Unchained labs Lunatic, etc.

Tarrytown, New York, Us

• Developed strategies to modernize and automate company-wide pathogen detection, sterility testing, and environmental monitoring• Researched alternative laboratory techniques and equipment globally to update company-wide facilities and methods • Wrote and revised Standard Operating Procedures (SOPs) for new and existing Quality Control tasks• Conducted environmental monitoring of processes areas to assist validation of analytical methods and techniques per US Pharmacopeia (USP), FDA, ICH, and Current Good Manufacturing Practice (cGMP) guidelines• Performed bioburden testing, LAL endotoxin kinetic turbidimetric assay testing, air monitoring, gram-staining, ELISA, and subculture growths using aseptic techniques• Utilized Laboratory Information Management System (LIMS) for sample input and test result tracking and analysis• Initiated Change Controls (CCs), Work Orders (WOs), and Purchase Orders (POs)• Collaborated in cross-department discussions of validation of new equipment and facilities• Maintained electronic laboratory notebook and logbook• Engaged with external vendors to manage procurement pipelines

Tarrytown, New York, Us

Tarrytown, New York, Us

• Researched over 30 GFP ELISA kits and successfully proposed alternatives for integration into lab protocol• Designed and constructed Electronic Lab Notebook (ELN) templates for company implementation• Gained experience with Hamilton Microlab STAR Liquid Handling System

College Park, Md, Us

• Guided weekly discussions for 25 students on foundational and contemporary scientific scholarship• Conducted Excel data analysis using large datasets to study avian sexual selection

College Park, Md, Us

• Facilitated Microbiology instructional lab for 40 students, explaining lab concepts and protocols• Trained undergraduate students on microscopy, plasmid sub-cloning, plate streaking, gram-staining, aseptic cell culture handling, transfecting, and cell-based assays

College Park, Md, Us

• Performed auditory discrimination tasks to measure neuro-auditory response properties in ferrets • Devised and conducted an independent research project yielding enhanced animal task performance

College Park, Md, Us

• Conducted research in the discovery of biophysical properties of ceramide voltage-gated channels• Constructed lipid bilayers and monitored various oscillators to stabilize the electric gradient

Houston, Texas, Us

• Conducted in vivo genotyping on over 1,000 human DNA and mouse tissue & blood samples• Analyzed sequencing data & successfully located nonsynonymous exonic variants for congenital defect • Performed molecular and cellular biological lab techniques for protein characterization like PCR, qPCR, ELISA, Western blot, SDS PAGE, NanoDrop (UV-vis) Spectrometry • Maintained HeLa and HEK mammalian cell lines for functional analysis

Tx, Us

•Analyzed ~200 CT Scans, measuring of fat, muscle, and bone content to gauge the effects of portal vein stents•Shadowed physician on clinical rounds

Houston, Tx, Us

• Monitored and performed lab duties related to vector construction, bacterial gel electrophoresis, PCRs, Western blot, ligations, transformations, cultures, sequencing, and CRISPR to assist in stem cell development pathway discovery

Master Of Science - Ms, Biotechnology

Bachelor Of Science - Bs, Biology/Biological Sciences, General

Education record