Ethan L. Ashley Email & Phone Number

San Diego, California, United States

• PI: Dr. Christian Metallo, Molecular and Cellular Biology Laboratory
• Independently design and implement tissue culture experiments to assess the effect of genetic perturbations to the metabolism of cancer cell systems
• Produce lentivirus particles and validate successful generation of stable cell line by stable isotope tracing
• Extract metabolites by liquid-liquid phase separation from tissue and cell culture samples for analysis by GC- and LC-MS
• Perform routine maintenance and troubleshooting on 3 tandem Agilent 7890-GC 5975-MS instruments
• Routinely handle mice for ultrasound measurement of orthotopic tumor burden

San Diego, California, United States

• Maintained HCT116, HaCaT, HEK 293T, and Suit-2 cell lines with sterile technique
• Analyzed CRISPR knockout efficiency of target proteins in HCT 116 cells using western blotting
• Quantified metabolic fluxes using stable isotopes, gas chromatography, and mass spectrometry
• Assessed anchorage independent growth of Suit-2 PDAC cells using soft agar colony formation assays
• Prepared 384-well plates to measure cellular ion channel activity with the Hamamatsu FDSS/μCELL
• Measured lipid synthesis of a doxycycline-inducible cell line using QQQ-LC/MS

San Diego, California, United States

• PI: Dr. Lingyan Shi, Laboratory of Optical Bioimaging and SpectroscopyProject: High Resolution Optical Imaging of Cancer Metabolism
• Performed a literature review of over 30 scientific journal articles on Raman spectroscopy, triple-negative breast cancer, and machine learning-based spectral analysis
• Collected 120+ Raman spectra over 8 weeks for 4 breast cancer subtypes using spontaneous Raman microscopy
• Worked as part of a team to develop a novel diagnostic pipeline for classifying TNBC subtypes with up to 95% accuracy using machine learning trained on Raman spectral data

San Diego, California, United States

• PI: Dr. Colleen McHugh, Department of Chemistry and Biochemistry
• Worked under limited supervision with graduate students to elucidate functions of long non-coding RNAs
• Executed techniques like DNA mutagenesis, PCR, and plasmid ligation to transform bacteria
• Utilized analytical techniques such as western blotting, EMSA, and RNA/DNA/protein gel electrophoresis
• Synthesized and purified recombinant protein using the AKTA pure protein purification system
• Documented my daily work and experimental results using the virtual notebook software Benchling

La Jolla, CA

• Delivered primary instruction during 10 weekly lab sessions to 9 freshmen bioengineering students
• Graded and provided feedback weekly to the 9 students assigned to my group
• Developed lecture materials and lab activities alongside the instructor and other TAs to be used to instruct the 195 students in the course