Ethan L. Ashley Email & Phone Number

San Diego, California, United States

PI: Dr. Christian Metallo, Molecular and Cellular Biology Laboratory• Independently design and implement tissue culture experiments to assess the effect of genetic perturbations to the metabolism of cancer cell systems• Produce lentivirus particles and validate successful generation of stable cell line by stable isotope tracing• Extract metabolites by liquid-liquid phase separation from tissue and cell culture samples for analysis by GC- and LC-MS• Perform routine maintenance and troubleshooting on 3 tandem Agilent 7890-GC 5975-MS instruments• Routinely handle mice for ultrasound measurement of orthotopic tumor burden

San Diego, California, United States

• Maintained HCT116, HaCaT, HEK 293T, and Suit-2 cell lines with sterile technique• Analyzed CRISPR knockout efficiency of target proteins in HCT 116 cells using western blotting• Quantified metabolic fluxes using stable isotopes, gas chromatography, and mass spectrometry• Assessed anchorage independent growth of Suit-2 PDAC cells using soft agar colony formation assays• Prepared 384-well plates to measure cellular ion channel activity with the Hamamatsu FDSS/μCELL• Measured lipid synthesis of a doxycycline-inducible cell line using QQQ-LC/MS

San Diego, California, United States

PI: Dr. Lingyan Shi, Laboratory of Optical Bioimaging and SpectroscopyProject: High Resolution Optical Imaging of Cancer Metabolism• Performed a literature review of over 30 scientific journal articles on Raman spectroscopy, triple-negative breast cancer, and machine learning-based spectral analysis• Collected 120+ Raman spectra over 8 weeks for 4 breast cancer subtypes using spontaneous Raman microscopy• Worked as part of a team to develop a novel diagnostic pipeline for classifying TNBC subtypes with up to 95% accuracy using machine learning trained on Raman spectral data

San Diego, California, United States

PI: Dr. Colleen McHugh, Department of Chemistry and Biochemistry• Worked under limited supervision with graduate students to elucidate functions of long non-coding RNAs • Executed techniques like DNA mutagenesis, PCR, and plasmid ligation to transform bacteria• Utilized analytical techniques such as western blotting, EMSA, and RNA/DNA/protein gel electrophoresis• Synthesized and purified recombinant protein using the AKTA pure protein purification system• Documented my daily work and experimental results using the virtual notebook software Benchling• Presented findings and progress from individual work up to twice a month at group meetingsWebsite: https://www.mchughlab.com/

La Jolla, Ca

• Delivered primary instruction during 10 weekly lab sessions to 9 freshmen bioengineering students• Graded and provided feedback weekly to the 9 students assigned to my group• Developed lecture materials and lab activities alongside the instructor and other TAs to be used to instruct the 195 students in the course