As a group leader, I manage of team of 8 people (scientists, lab technicians, PhD students) to develop our skills and expertise in synthetic biology applied to biobased chemistry. We aim at developing new metabolic pathways for the production of bulk chemical compounds from renewable resources.Personnal skills : project management, design of metabolic pathways, phylogenetic analysis of enzyme families, design of libraries, robotic platform programming.Group skills :• Design… Show more As a group leader, I manage of team of 8 people (scientists, lab technicians, PhD students) to develop our skills and expertise in synthetic biology applied to biobased chemistry. We aim at developing new metabolic pathways for the production of bulk chemical compounds from renewable resources.Personnal skills : project management, design of metabolic pathways, phylogenetic analysis of enzyme families, design of libraries, robotic platform programming.Group skills :• Design : computational protein design, genome scale metabolic models with thermodynamic constraints ;• Enzyme engineering : in vivo evolution of enzymes using dedicated screening strains, in vitro directed evolution of enzymes, high-throughput screening using robotic platforms ;• Molecular Biology : DNA assembly for combinatorial generation of enzyme libraries and metabolic pathways, targeted genetic and metabolic engineering in E. coli and S. cerevisiae using CRISPR/Cas9,• Biochemistry : heterologous enzyme production and purification, biochemical characterization, implementation of enzyme cascades. Show less

As a Scientist, I work in fermentation technology using filamentous fungi (Trichoderma, Aspergillus, Rhizopus) and yeasts (S. cerevisiae, P. pastoris) for the production of either enzymes or chemical compounds, in both cases for the upgrading of lignocellulosic biomass in biofuels and biobased chemicals. Our research is either fundamental, to better understand of the physiology of these strains, or applied, to design economically viable biorefineries.Main activity : Optimisation of… Show more As a Scientist, I work in fermentation technology using filamentous fungi (Trichoderma, Aspergillus, Rhizopus) and yeasts (S. cerevisiae, P. pastoris) for the production of either enzymes or chemical compounds, in both cases for the upgrading of lignocellulosic biomass in biofuels and biobased chemicals. Our research is either fundamental, to better understand of the physiology of these strains, or applied, to design economically viable biorefineries.Main activity : Optimisation of fermentation using filamentous fungiSkills : aerobic fermentation at scales ranging from 100µL to 10 L, optimisation of culture conditions in batch, fed-batch or continuous modes, phenotypic characterization of genetically improved strains, integration of industrial products or waste from lignocellulosic biomass transformation process, morphology engineering, Secondary activities, with colleagues experts in the field :• Genomics and transcriptomics of Trichoderma reesei strains• Scale-up, mixing and oxygen transfer in industrial bioreactors, rheology of viscous fermentation broth• Measurement and application of cellulase activities, lignocellulose hydrolysis, Simultaneous saccharification and fermentationSide activity : RoboticsTo increase our efficiency, I programmed our two robotic platforms (colony picker and liquid handling) to transfer our manual protocols : sample preparation for chromatography, protein assays, enzyme assays, colony picking, strain library management, parallel microscale cultivation, protein expression and cell lysis, directed evolution, HisTag protein purification, ... Show less

PlayGuide is a web app for parents to find nearby playgrounds, developed in our spare time with a father friend. Available at playguide.eu and on Google Play.