In my thesis, I combine systems biology, data science, metabolic engineering, and dynamic optimization knowledge and techniques to address gaps in our understanding of small-molecule regulation of metabolism in three different projects:1. Construction and structural analysis of genome-scale small-molecule regulatory networks to understand how they function and evolve2. Modeling and optimization of small biochemical networks to predict optimal control systems in … Show more In my thesis, I combine systems biology, data science, metabolic engineering, and dynamic optimization knowledge and techniques to address gaps in our understanding of small-molecule regulation of metabolism in three different projects:1. Construction and structural analysis of genome-scale small-molecule regulatory networks to understand how they function and evolve2. Modeling and optimization of small biochemical networks to predict optimal control systems in various metabolic contexts3. Construction of novel metabolic-and transcriptional-level controllers to characterize and compare open-loop control systems in real metabolic systems Show less

Honours thesis student working under the supervision of a PhD student to validate a multistate computational protein design approach• Optimisation of a protein expression and purification protocol• Development of a protein stability assay for 34 engineered protein variants• High throughput validation of engineered variants for stability, conformation, and dynamics• Data analysis using non-linear regression and ANOVA

Thesis student working under a PhD student on a synergistic lysis project:• Model generation through statistical analysis of previously-gathered protein extraction data • Prediction of protein yield and specificity for synergistic cell lysis using detergent(s) and heat• Design of protein extraction experiments to complete this model

Worked within a team of undergraduate and graduate students to conceptualize and carry out three different Synthetic Biology projects as part of the International Genetically Engineered Machines (iGEM) Competition hosted by the Massachusetts Institute of Technology (MIT):• Generation of a bistable genetic toggle switch in yeast• Creation of a standardized process for the rapid creation of genetic constructs in a yeast chassis• Modification of an endemic probiotic for the conversion… Show more Worked within a team of undergraduate and graduate students to conceptualize and carry out three different Synthetic Biology projects as part of the International Genetically Engineered Machines (iGEM) Competition hosted by the Massachusetts Institute of Technology (MIT):• Generation of a bistable genetic toggle switch in yeast• Creation of a standardized process for the rapid creation of genetic constructs in a yeast chassis• Modification of an endemic probiotic for the conversion of dietary sugar into fibre for us as a novel blood sugar regulation system Show less