Protein engineering to enhance thermal and pH stability for the development of sustainable fibers

• Engineered fluorescent proteins to enhance thermal and pH stability for the development of sustainable fibers• Built protein libraries with molecular cloning• Optimized production conditions of recombinant proteins, expressed them in E. coli, and purified them with Ni-NTA affinity chromatography• Characterized proteins with UV-Vis spectrophotometry, Qubit fluorometry, and SDS-PAGE

• Engineered 15 disordered polypeptides with hydrophilic-charged block architecture for intracellular delivery• Produced recombinant protein with bacterial expression in E. coli and purified protein using AKTA FPLC• Characterized polyelectrolyte complex micelles by turbidity assay and DLS measurement• Analyzed protein assemblies using LCMS, SDS-PAGE, Bradford assay, BCA assay, and Western Blotting

• Expressed and purified cell wall binding domain (CBD) of cell lytic enzymes that induce signal for bacterial detection• Analyzed protein through SDS-PAGE, Native PAGE, Bradford Assay, UV-Vis Spectrophotometry, and flow cytometry for characterization• Evaluated catalytic activity, antimicrobial activity, enzyme stability, and reusability with colorimetric assays and cell-based assays for enzyme-immobilized composite coatings of polydopamine and perhydrolase, generating potent disinfectant

• Created, revised, and proofread documents of more than 100 prescription drugs in Quality Assurance Team• Assured regulatory compliance with the Ministry of Food and Drug Safety (MFDS)