Evi Lim Email & Phone Number

United States

Madison, Wisconsin, United States

- Assisted senior scientists on optimizing NGS application (ex: Miseq-Illumina) and implementing the newly acquired high-throughput platform (ex: Beacon-Berkeley Light), specifically on handling downstream processes to minimize degradation risk.- Performed thorough literature search on new innovations and well-thought strategies on projects with high variable determinants.- Demonstrated creative thinking and troubleshooting capability on complex experimental design. - Drafted SOP, provided training and oversight to other functional group on single cell encapsulation workflow in microfluidic platform (Nadia Instrument, Dolomite).- Gained proficiency on single cell RNA isolation and purification, first strand cDNA synthesis (RT-PCR) and total cDNA amplification from isolated B-cells.- Explored ribosome and mRNA display technology using reconstituted cell free expression system.- Proficient in gene editing softwares (ex: SnapGene), including primers design, inserts construction, vector linearization, light- and heavy-chains fragment assembly, sequencing comparison and validation.- Hands-on experience on bispecific and trispecific antibody engineering, subcloning strategies and assembly process for new constructs with multi-binding capability to different target molecules.- Assisted Discovery on projects with accelerated timeline, including phage preparations and precipitations, ELISA(s), colony PCR sequencing, reformatting binders for transfection, kinetics measurement (Octet) and other related workflow.

Madison, Wisconsin, United States

- Routinely cultured, expanded and maintained human induced pluripotent stem cell (hiPSC) in defined medium.- Maintained accurate records of experiments and documented hiPSC cultured in daily progression.- Trained on hiPSC differentiation into functioning beta cells, islet like cluster (ILC).- Provided mechanical and technical expertise on imaging system to track hiPSC proliferation and differentiation progress.- Performed Quality Control of differentiated ILC product via Flow Cytometry, Glucose Stimulating Insulin Content (GSIS), Insulin Index and ELISA assay.

Madison, Wisconsin

Glukhov Lab - CMARP (Cellular and Molecular Arrhythmia Research Program)- Assisted lead scientists on Western blot, Transmission Electron Microscopy (TEM), cell/tissue staining, data statistics and analysis on several successfully published research journals.- Coordinated region specific tissue cryogenic collection from multi-species and kept detailed inventory to track sample distribution on multi-experimental studies. - Allocated bench-top equipment and oversaw instrument and gene editing software installation for workstations in a newly set lab facility.- Trained undergraduate students on animal husbandry and handling, laboratory safety, including proper handling of liquid nitrogen, stock buffer preparation, and chemical hygiene practices.

Madison, Wisconsin Area

- Part of R&D team developing novel and sustainable biocontrol agents to counteract infections caused by antibiotic-resistant bacterial disease in crops, animals and human.- Initiated and led projects to revive soil amoebas, such as Dictystelid discoidium (dicty) variants collected worldwide.- Documented and presented findings on interactions between dicty and its ability to feed upon various biofilms. - Utilized sterile tissue culture techniques working with pathogens known to cause disease in human and plants.- Implemented SOP and chemical hygiene in compliance with NIH and USDA research regulations and guidelines.

Madison, Wisconsin Area

Cardiovascular Research Center (CVRC)Cellular and Molecular Arrhythmia Research Program (CMARP)Principle Investigator (PI): Craig T. January MD. PhD.- Extensively trained in electrophysiology recording from stably expressing cell lines and isolated cardiomyocytes at physiological temperatures for drug response studies on hERG (potassium channel in heart) channels.- Managed stock inventory, documentation, distribution and provided technical support to pharmaceutical (worldwide) and academic institution requests on patented hERG stable cell line.- Proficient in cellular and subcellular image implementation (i.e. confocal, on-cell licor/odyssey).- Trained junior lab members on mammalian cell culture techniques and cryopreservation.- Assisted scientists and graduate students on literature, methodology and protocol optimization.Engineered constructs expressing mutant hERG from patients with Short QT Syndrome and Long QT Syndrome.- Experienced in High Throughput Small Molecule screening assay to screen FDA-approved drugs for potential arrhythmia-induced effects and to identify compounds with potential for novel therapeutic application.- Routinely screened mammalian cell line stably expressing wild-type or mutant hERG through western blot and patch clamp method.- Implemented complex qualitative analysis and presented findings at weekly held journal meetings.- Supervised undergraduate students on media preparation, lab bench techniques and chemical hygiene.