Subject: Understanding the role of the fetal liver microenvironment in the developing hematopoietic system. Developed multi-color (>20 markers) flow cytometry panels for characterization and cell sorting of hematopoietic cells and their microenvironment for single-cell molecular profiling and 2D and 3D cell co-culture.. In vitro modeling of the fetal liver microenvironment to probe the contribution of specific stromal cells to the maintenance and expansion of the hematopoietic system. . Implemented tissue architecture analysis using clearing and confocal microscopy of hematopoietic/immune tissue. . Collaborated with industry leaders to develop a spectral flow cytometry analysis pipeline that significantly enhances cell subset identification in complex samples. . Collaborated with physicists and bioinformaticians to devise a machine-learning image analysis pipeline, enabling single-cell network characterization of complex tissue microscopy images. . Represented and advocated for postdoctoral researchers, actively involved in communication with leadership to influence decisions in favor of postdocs, and skillfully organized events. Achievements: 1 publication, 1 oral communication and 2 posters in international conferences; collaborations: 1 internal and 2 external; 1 research grant

Subject: Elucidating the waves of hematopoietic cell production and their contributionto the establishment of the adult hematopoietic system Key Responsibilities:. Devised multi-color (>12 markers) flow cytometry panels that allowed the ex vivo identification of a subset of developmentally restricted erythroid progenitors that were subsequently profiled through single-cell gene expression analysis and for lineage potential using in vitro cultures. .Performed cell cycle analysis and cytokine sensitivity assays to study the selective advantage of these cells over hematopoietic stem cell derived progenitors..Developed Basic R programming algorythms for the analysis of high-dimensional flow cytometry data and single-cell gene expression.. Conceived and delivered comprehensive lectures and practical courses on hematopoietic stem cell biology.Achievements: 5 publications, 8 oral and 6 poster communications in international conferences; 2 internal collaborations; management of 1 student

ATG (All Time GABBAs) is a non-profit organization gathering previous and current students of GABBA, as well as professors involved with the program and all who had or have a relationship with GABBA.ATG was created to develop a community spirit expressed in the sentence "once a GABBA always a GABBA", where responsibilities should be shared and an active team effort should be in action to foster the program growth and ultimately benefit all members.Specific Roles:- Design and launch of science outreach/educational initiatives (Maria de Sousa Summer Research Program - launched in 2017)- Organization of scientific symposiums - Fundraising

Subject: Identification of a gene involved in cardiac specification during development using embryonic stem cell in vitro differentiation systems