Fabian Hia Email & Phone Number

Singapore

Singapore

Established multiple mammalian cell lines expressing commercial proteins, integral to a pipeline for re-engineering and enhancing cellular translational capabilities. Assisted in developing a cell-line-based cancer drug discovery platform for massively parallel screening of chemical libraries against undrugged cancers. Contributed in development of a bioinformatic pipeline for evaluation of NGS results derived from massively parallel screens.

Assessed and implemented a NGS bioinformatic workflow by assessing and comparing different iterations to establish local best practices.

Experienced in conducting transcriptome analysis for knockdown cell lines, and comprehensive analysis of oncogenes and tumor suppressor genes. Utilized cancer patient databases (ICGC, TCGA) for hazard ratio assessments and survival curve evaluations. Proficient in data mining (DEPMAP) for essentiality and disease associations in cancer cell lines. Skilled in pathway and gene ontology analyses of codon-enriched oncogenes and proto-oncogenes.

Kyoto, Japan

Developed cell sorting-based CRISPR-Cas9 screening and fine-tuned codon optimality regulation techniques. Accomplished in utilizing next-generation sequencing, ribosome profiling, and gene set enrichment analysis to identify key pathways and regulators. Investigating RNA binding mechanisms and RNA-protein interactions. Integrating bioinformatics, biostatistics, and wet-lab data for hypothesis generation and target identification. Performed collaborative interdisciplinary projects focusing on… Show more Developed cell sorting-based CRISPR-Cas9 screening and fine-tuned codon optimality regulation techniques. Accomplished in utilizing next-generation sequencing, ribosome profiling, and gene set enrichment analysis to identify key pathways and regulators. Investigating RNA binding mechanisms and RNA-protein interactions. Integrating bioinformatics, biostatistics, and wet-lab data for hypothesis generation and target identification. Performed collaborative interdisciplinary projects focusing on RNA regulation, immune response, and therapeutic intervention. Presented complex computational data to diverse audiences. Show less

Kyoto, Japan

Building on post-graduate PhD project. Verified transcriptome-wide codon optimality bias using advanced sequencing and statistical techniques. Conducted ribosome and disome profiling to study the role of RBPs in ribosome stalling and collision. Utilized gene set enrichment analysis to identify pathways in knockdown/out cells. Generated dominant-negative helicase-dead mutant helicases for investigating RNA binding mechanisms of DEAD-box helicase DHX29 in vitro. Integrated bioinformatics… Show more Building on post-graduate PhD project. Verified transcriptome-wide codon optimality bias using advanced sequencing and statistical techniques. Conducted ribosome and disome profiling to study the role of RBPs in ribosome stalling and collision. Utilized gene set enrichment analysis to identify pathways in knockdown/out cells. Generated dominant-negative helicase-dead mutant helicases for investigating RNA binding mechanisms of DEAD-box helicase DHX29 in vitro. Integrated bioinformatics, biostatistics, and wet-lab data for RBP hypothesis generation and target identification. Prepared concise presentations of computational data for diverse audiences.Collaborated on interdisciplinary projects, including bioinformatics analyses of RNAseq and RNA-immunoprecipitation to elucidate therapeutic pathways for inflammation. Conducted transcriptomics data modeling to infer ligand-receptor activities in pulmonary arterial hypertension. Analyzed RNA-binding protein motifs and performed bioinformatics analysis on transcriptome-wide RNA structure dynamics in response to LPS stimulation. Show less

Kyoto, Japan

Kyoto, Japan

Singapore

• Isolated and purifying individual RNA species using High Performance Liquid Chromatography(HPLC) and size exclusion columns• Developed and optimized a solid-phase RNA extraction method recover the entire spectrum of non-coding RNA from Mycobacterium bovis (BCG)• Assisted in optimizing a method for the purification of individual non-coding RNA species from Mycobacterium bovis (BCG) using HPLC 2-dimensional size-exclusion techniques• Documented procedures and prepared orders and… Show more • Isolated and purifying individual RNA species using High Performance Liquid Chromatography(HPLC) and size exclusion columns• Developed and optimized a solid-phase RNA extraction method recover the entire spectrum of non-coding RNA from Mycobacterium bovis (BCG)• Assisted in optimizing a method for the purification of individual non-coding RNA species from Mycobacterium bovis (BCG) using HPLC 2-dimensional size-exclusion techniques• Documented procedures and prepared orders and quotations for the group• Prepared and maintained inventory of parts and supplies for the Mass spectrometry facility Show less

Basel Area, Switzerland

Masters of Science in Infection Biology. • Established, characterized, and optimized the infection of THP-1 cells by Mycobacteriumbovis BCG as part of a lipidomics study on mycobacterial infection• Characterized the immune responses of the human THP-1 macrophage-like cell line toLipopolysaccharide and Kdo2 Lipid A• Investigated growth dynamics of Mycobacterium bovis BCG in non-hydrolyzable detergentTyloxapol as an alternative to Tween 80

Singapore

Doctor Of Philosophy (Ph.D.)

Mext Research Student

Master'S Degree, Infection Biology

Master'S Degree, Infection Biology

Bachelor Of Science, B.Sc. (Hons), Life Sciences