Fabrice Plaisier Email & Phone Number

I am a PhD level scientist in Neurosciences with over 10 years post-doctoral experience in both academia and biological industry. I am particularly interested in carrying out biomedical projects in the fields of neurosciences, physiology or neuroendocrinology. I particularly enjoy collaborating with scientists from different disciplines to develop new skills and solve new challenges.My general skills are:- Autonomy, adaptability, organisation, planned work to achieve goals and targets on time- Work as a team member and under internal control procedures- Teaching, supervision of students, adapting to different scientific levels and backgrounds- Preparation of reports and scientific articles, dissemination of scientific informationPersonal profile:Neurosciences, physiology, stroke, neurovascular coupling, ion channel drug discovery, stroke, circulation, vascular smooth muscle cells, pharmacology, culture cells, ion channels, patch-clamp, screening, homeostasis and energy balance, neuronal tracing, confocal microscopy.Teaching experience (tutorials and practical works of animal physiology).Experimental skills:ELECTROPHYSIOLOGY-Whole-cell/Loose patch-clamp in rodent brain slices or freshly dissociated cells (vascular smooth muscle cells from cerebral arteries) or stably/transitory recombinant cell lines (HEK, CHO, U2OS)-Automated Patch clamp system (QPatch, Sophion)-Patch-clamp with Dynaflow® Resolve system-Functional Drug Screening System by fluorescence (FDSS, HAMAMATSU)-Ionic conductances covered: Kir/Kv/GABAergic/5-HT/P2X channelsExperimental skills:SURGERY - HISTOLOGY – BIOCHEMISTRY – MOLECULAR BIOLOGY-Rodent handling (IP injection, blood collection, organ collection, small surgery), management of transgenic rat colony-Stereotactic injection of neuronal tracers or AAV in rodent brain using iontophoretic or pressure injection systems-Histology (whole animal perfusion fixation, brain sectioning, embedding in paraffin, freezing microtome)-Confocal microscopy (Nikon A1R Fluorescence Life time Imaging Microscope)-Biochemical and Molecular techniques (Protein determination: BCA protein assay, SDS-PAGE, immunohistochemistry and fluorescence, single-cell RT-PCR, genotyping)-Good notions of vascular myography-Behavioural tests (sensorimotor assessment after cerebral ischemia-reperfusion injury in rats)-Computer skills: Microsoft Office, pCLAMP (Clampfit, Clampex), GraphPad Prism, SPSS, Origin9, Image J / IMARIS microscopy image analysis software

Listed skills include Pharmacologie, Neurosciences, Rt Pcr, Laboratoire, and 10 others.

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  Scientific Researcher

  Inovarion

  Paris, Fr