Fabrizio Lecce Email & Phone Number

Turin, Piedmont, Italy

The core activities of my Team (1 scientist + 2 technicians + 1 PhD student) are environmental monitoring by traditional Sanger sequencing and the Monocyte Activation Test (MAT). By implementing the MAT, we will provide an in vitro, faster, and ethically driven alternative to the rabbit pyrogen test (RPT). A slight increase in associated costs will be.

Turin Area, Italy

I was responsible for the BQC - Molecular Biology Biosafety Lab (2 scientists+3 technicians). The most relevant activities were the detection of potential MVM, Vesivirus and Mycoplasma contaminations by Real-Time PCR methods and environmental monitoring by Sanger sequencing. An automated sample preparation for the environmental monitoring was crucial to.

Turin Area, Italy

- Team Leader of the Next Generation Sequencing (NGS) based project with the aim of replacing traditional Sanger sequencing to qualify Merck cell banks. Such a project was also my PhD project in Complex Systems in Life Sciences. My PhD program began in January 2014.- Molecular Biology Group Responsible for the implementation of a Laboratory Information.

Turin Area, Italy

I was involved in the Mycoplasma Global Project. The purpose of this project was the replacement of the traditional methods to detect potential Mycoplasma contaminations with a NAT method (Nucleic Acids Techniques based method). We developed a Real Time PCR based method in accordance to the Health Authorities requirements.

- Nucleic acid sequence determination of the protein-coding sequence and the 5’ and 3’ flanking control regions by Sanger sequencing.- Determination of Mycoplasma contamination by end-point PCR in internal cell lines as in process control.

Type of sector: Environmental ResearchResearch Grant: detection of GMO by means of Real Time techniques in according to EMA guidelinesMain activities and responsibilities: - GMO in animal feeds, bacteria and virus detection of animal diseases by real-time PCR- DNA and RNA extraction from difficult matrices

Type of sector: Environmental ResearchBioremediation: development of remediation methods based on the use of natural degradation in the respective environmental compartmentsI gained hands-on experience with Denaturing Gradient Gel Electrophoresis (DGGE) to monitor shifts in bacterial populations within soil samples.

Type of sector: Environmental Research“Leonardo da Vinci “ research fellowship arranged by University of Rome "Sapienza": Assessment of in situ Trasformation of Xenobiotioc Organic MaterialThe aim of the project in which I was involved was the development of a method to detect P. putida and T.aromatica and study of their genetic expression

Master'S Degree, Advanced Technologies Applied To Biotechnologies

Single Cycle Honors Degree, Biotechnologies

Doctor Of Philosophy - Phd, Complex Systems In Life Sciences

Development of Next Generation Sequencing tools for cell bank characterization in Biopharmaceutical Production