Bioinformatics Scientist
CurrentBioStudio:- Managed all notebooks within the BioStudio platform, creating comprehensive and accessible tutorials for diverse bioinformatics methods including scRNA-seq, Bulk RNA-seq, and Spatial RNA-seq.- Isolated each notebook from its environment, resolving conflicts between R and Python packages, and providing corresponding data samples. Successfully handled multiple CUDA versions for notebooks utilizing GPU frameworks like TensorFlow, PyTorch, and AlphaSC, ensuring environment compatibility.- Created pipelines for running large-scale data analyses from Seurat (R) datasets with InferCNV and CellChat. These pipelines were implemented as tool scripts, allowing users to input datasets via the command line and automatically obtain all analysis results from the packages.Research in Cell-type Prediction:- Implemented memory lazy loading to handle massive-scale datasets exceeding RAM constraints during training. - Leveraged Variational Autoencoder models (scVI, scANVI), and attention-based models (scGPT, Geneformer) to enhance cell type prediction accuracy and integration performance. - While these models effectively mitigated batch effects, accurate prediction of rare cell types remains a challenge due to the inherent overdispersion and heterogeneous of scRNA-seq data, which is crucial for effective disease treatments.Research in Cell Instance Segmentation:- Researched and implemented a custom U-Net model from scratch, inspired by the Cellpose architecture for image instance segmentation. - Optimized model performance through architectural modifications, achieving a 3% improvement in mean Average Precision (mAP) compared to the original Cellpose. Accelerated inference by deploying the model using TensorRT, resulting in a 2x speedup. - While the model demonstrated improvements in specific scenarios, achieving over 90% accuracy remains crucial to fully meet the project’s requirements, as cell instance segmentation impacts all downstream analyses.