Dissertation Topic: Characterization of the crosstalk between TNF-α and TWEAK/Fn14 axis in the rheumatoid arthritis (RA) synovial fibroblasts and to decipher non-responsiveness to anti-TNF therapy.• Designed, conducted, and interpreted experiments on primary synovial fibroblasts to study cytokine biology using molecular biology techniques.• Researched the molecular mechanism behind the non-responsiveness to TNF inhibitors in RA patients using primary synovial fibroblasts and clinical samples from RA patients.• Presented the potential cause of suboptimal effectiveness of TNF-α drugs at the Industrial Advisory Board Meeting, WSU.• Supervised and trained three Ph.D. rotation students and one summer intern on several molecular techniques, data analysis, data interpretation, and proper lab safety guidelines.• Contributed to research on multiple ongoing lab projects on inflammation and rheumatic diseases and substantially added to the lab’s ongoing grant research project on microRNA.• Wrote and reviewed grants and IACUC protocols and managed the ordering of resources, including chemicals and reagents for the lab.• Collaborated with clinicians and biostatisticians.• Presented research at annual graduate seminars and participated in weekly lab meetings and discussions.

• Isolated peripheral blood mononuclear cells (PBMCs) and granulocytes from human and rodent blood and bone marrow• Conducted antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) bioassays • Utilized Flow Cytometry to assess the immune engaging functions of bispecific antibody affinity variants

• Proficiently performed quality control analysis of therapeutic and non-therapeutic molecules using a wide range of techniques, including HPLC, UPLC, CIEF, Western Blotting, glycan content analysis, IEF, 2D-electrophoresis, Ion Exchange Chromatography, Gel Filtration Chromatography, and IMAC.• Developed & validated analytical methods for drugs as per United States Pharmacopeia (USP) & European Pharmacopoeia (Ph. Eur.).• Conducted instrument calibration to ensure accurate and reliable results.• Prepared comprehensive documentation, including SOPs, MOAs, study protocols, and reports.• Successfully facilitated technology transfer to the manufacturing site.• Conducted drug stability testing to assess their shelf life and ensure product efficacy.

Research and Development Dept. (Native Proteins) • Conducted independent research on retinol-binding protein and purified it from renal failure urine in less than six months

• Overexpressed r19kDa/ LpqH protein after induction of recombinant plasmid with IPTG• Purified recombinant 19kDa protein using Ni-NTA Chromatography technique• Handled Animal Tissue Culture Techniques using Raw264.7 cell line & Studied induction of autophagy by chemiluminiscence