HoloBiotics srl è una startup innovativa e spinoff dell’Università di Napoli Federico II. HolobBotics fornisce consulenza per lo sviluppo di prodotti e soluzioni bio-based, metaboliti prodotti da microorganismi wild-type, ceppi microbici e fungini, composti naturali e soluzioni biotecnologiche per processi industriali più sostenibili. Ci riferiamo ai settori della salute e dell'ambiente, della cosmetica e della detergenza.

International Human Microbiome Coordination and Support Action Project - H2020 Grant agreement ID: 964590

▪ Formulation of Industrial Cleaning Products.▪ Development of green biotech, biological (bacterial spores) and enzymatic detergents.▪ Contacts with distributor; production of technical and security sheets.▪ Formula upgrades, quality control (microbiological and physical) testing of new products

Microbiology and Hygiene Labs: Waters, Food, and EnvironmentR&D Project Management & Quality Assurance: food, beverages, API, drugs, detergents, sludges.Most recent R&D Projects:▪ Innovative enzymatic clean in place technologies in food companies biological productions. [POR CAMPANIA FESR 2014/2020 – CUP:B83D18000460007].▪ Bottled water microbiota: characterization and comparison between sparkling and flat waters. ▪ Metagenomic analysis of anaerobic digestate for the performance enhancement of the product.▪ Mitigation of soil liquefaction potential by injection of non-pathogenic bacteria to generate gas bubbles as final product of their own metabolism. [POR CAMPANIA FESR 2014/2020].▪ Formulation of bactericides and for Legionella spp. treatment of hospitals and hotels.▪ Thermal Mud safety and quality.▪ Tea and Infusions microorganisms molecular identification via DNA barcoding.▪ Monitoring system for the identification and enumeration of new microbial species [Aquasystem Project: O.R. 2.3 - PON 2007-2013].▪ Microbiome and antibiotic resistome in treated wastewater [Nereus COST Action WG1].▪ Sourdough Certification: microbiological and physicochemical properties of sour dough; characterization of microbial communities responsible for fermentation.▪ VOCs as markers of microbial contamination of food: use of SPME-HS-GC/MS for the detection of pathogens from meat and milk.▪ Dairy industries emerging pathogens.▪ Salmonella spp. in meat from slaughterhouses.▪ Other: competences in EU-GMP, CFR 21, ISO, REACH, CLP, ISO/IEC 17025:2005.

Molecular Biology applied to food analyses:▪ DNA extraction from wine and characterization of prevalent population of yeast and bacteria;▪ Evaluation of the minimum detectable microbial load in commercial food and beverages via qPCR;▪ Analysis of terminal restriction fragment length polymorphisms (T-RFLPs), gene sequencing for animal differentiation applied to food. Proteomics applied to food analyses:▪ Instrumental Protein Analysis: proteins extraction methods, SDS Page, identification of proteins/proteome via UPLC-MS/MS of selected sample-material (crops, feed, food, other plant-materials), identification of target peptides for quantification.Chemical Analysis for Metabolomics approach in food matrices: ▪ screening for potential natural colorants (anthocyanins) extracted from fruit juices, structure determination/verification (HPLC-MS/MS), purification;▪ Volatiles monitoring in vegetable-storage devices: establishment of reference analysis via HS- GC-MS, identification of target volatiles suitable for electrochemical detection (ECD), recording of volatile kinetics.Environmental Chemical Analyses ▪ Determination of Daphnia magna fatty acids composition via GC-FID after ecotoxicological evaluations;▪ Patterns, levels, and trends analysis of perfluorinated compounds (PFC) in food contact materials and other consumer products via LC-ESI-MS/MS.

▪ Quality control of products: respect of processes and maintenancewithin product development.▪ Microbiological, analytical, and physical testingof cosmetics.▪ Microbial safetyevaluations(according to ISO protocols).

▪ Monitoring of retail products and characterization of isolated microbial species (virulence factors, ability to form biofilm, antibiotic resistance).▪ Microbiological analyses of food and waters (according to ISO protocols); ▪ Monitoring of food chains for the elaboration, control and evaluation of HACCP method; ▪ Selex ES SpA Project: microbiological analysis of waste waters from Fusaro and Giugliano (NA) facilities (Finmeccanica Group) for the elaboration of guidelines to realize adequate wastewater treatment strategies. Determination of wastewaters toxicity (Daphnia magna test). Evaluation of the biological performance of activated sludge: sludge biotic index (SBI);▪ Epidemiologic investigations.

▪ Microbiological and chemical analysis of several biological specimens▪ Legionellosis Risk Assessment▪ Epidemiological investigations.

▪ Waters microbiological analysisand testing of new techniques for faster environmental detection of Legionella pneumophila(Legipid® Legionella Fast Detection Kit –Biotica).▪ Microbiological Analysis of Foods:official cultural methods for the identification of the most common pathogens in foodand beverage products.▪ HACCP and Quality Assurance: coordinator for the HACCP program.▪ Microbiological and Chemical Analyses of mineral, waste and thermal waters.