Federica Poletti Email & Phone Number

Milano, Lombardia, Italia

- Managment and coordination of people in Cell Factory Unit with activity of cell culture and solutions preparation- Responsible of coordination cell lines provisions for Assay Development Unit, HTS Unit, Compound profiling Unit , High Content Immunofluorescence Unit, and Ephys Unit- Studying new cell lines income in Axxam both from clients and internal unit to guarantee MB and WB cells generation with respecting QC - Responsible for all cell factory managerial tasks including ordering, ensuring stock maintenance, administering cell lines repositories- Supervision on preparation of solutions - Supervision and coordination internal references for LN2 Big container and Cell Lines Assay Database- Collaboration with Business department people to proposal preparation- Collaboration with Purchasing and Logistic to improve order and quality of material useful for Cell Culture- Development of Standard Operating Procedures (SOP) in collaboration with QA,Facility Manager and HSE- Implementation of Unit assets selection of skilled people

Bresso (Mi)

- Mammalian cell culture handling: seeding, growing, thawing and freezing technics and cell culture media preparation- Insect cell culture handling: seeding, growing, thawing and freezing technics - Cell banking for the Axxam Cell lines/Assays repository of MB and WB with respect of QC- Cell culture expansion and seeding for ephys analysis- Cell handling in large scale format and plating in 384 MTP for HTS purpose compound profiling and hit to lead purpose, by using highly automated dispending devices. (Multidrop and Matrix Well Mate)- Cell culture transient transfection and plating in 384 MTP for HTS.- Cell culture differentiation and plating in 384 MTP for HTS.- Preparation of solution and buffer for the whole Company

Caponago

- Perform manufacturing operation in clean room with hybridoma cell lines, following GMP requirements- Develop a cell based QC test with FACS

Milano, Italia

Maintenance of normal, primary, tumoral, stem cells, induced pluripotent stem cells (human, murine) cell cultures. Cell bank authentication and quality control with molecular biology and biochemical techniques (STR profile, mycoplasma detection). Cell engineering and gene expression analysis. Molecular cytogenetic techniques. Training to work in sterility in BSL1 and BSL2 rooms

Laboratorio Di Biologia Delle Cellule Staminali Diretto Da Prof. Elena Cattaneo

Development of a protocol for the differentiation of human embryonic stem cells towards a neuronal fate.Maintenance of cell cultures, in particular human embryonic cell cultures and their feeders (murine embryonic fibroblasts). Extraction of RNA and DNA from tissue and cell samples. Gene expression analysis by RT-PCR and PCR. Immunofluorescence analysis.FACS analysis and good knowledge of confocal microscopy.

Laurea Specialistica, Biotecnologie Del Farmaco

Laurea Triennale, Biotecnologie Farmaceutiche

Perito Chimico, Indirizzo Ecologico-Sanitario, Perito Chimico Ecologico Sanitario

Marzo 2004: Tirocinante presso il Laboratorio analisi (sezione elettroforesi e microbiologia) dell'ospedale Bolognini di Seriate