-Developed, optimized and validated a novel ANGPTL8 ELISA to measure circulating A8 levels in the Dallas Heart Study.Skill sets: assay development, optimization and validation according to the Clinical and Laboratory Standards Institute (CLSI) and the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH), assay execution, plasma samples handling and statistical analysis.- Characterized Angptl8 liver and adipose-specific knock-out… Show more -Developed, optimized and validated a novel ANGPTL8 ELISA to measure circulating A8 levels in the Dallas Heart Study.Skill sets: assay development, optimization and validation according to the Clinical and Laboratory Standards Institute (CLSI) and the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH), assay execution, plasma samples handling and statistical analysis.- Characterized Angptl8 liver and adipose-specific knock-out mice Skill sets: mouse handling, VLDL secretion, lipid, glucose, insulin and fatty acid measurements, indirect calorimetry, body composition (NMR), immunoblotting and immunoprecipitation, adipocyte culture and differentiation, viral infections and statistical analysis. Show less

- Characterized the first functional mutations in LRP1 in humans. Skill sets: CRISPR/Cas9 genome editing and siRNA knockdown in human cell lines, human fibroblasts culture, protein stability, biotinylation assay, immunoblotting, immunofluorescence, molecular cloning, targeted quantitative proteomics, qRT-PCR and statistical analysis.- Demonstrated that complete and partial LCAT deficiency is differentially associated with atherosclerosis. Skill sets: statistical… Show more - Characterized the first functional mutations in LRP1 in humans. Skill sets: CRISPR/Cas9 genome editing and siRNA knockdown in human cell lines, human fibroblasts culture, protein stability, biotinylation assay, immunoblotting, immunofluorescence, molecular cloning, targeted quantitative proteomics, qRT-PCR and statistical analysis.- Demonstrated that complete and partial LCAT deficiency is differentially associated with atherosclerosis. Skill sets: statistical analysis.- Determined the ability of gene and metabolic risk scores to predict hypertriglyceridemia and cardiovascular disease risk. Skill sets: gene and metabolic risk scores, association studies and statistical analysis. Show less