Conducted detailed enzyme characterization by various methods.Developed improved expression and purification procedures for several recombinant enzymes (including the challenging Fe2+-dependent, poorly expressed, insoluble, cysteine-rich dioxygenase).Identified and fixed a major analytical flaw caused by enzyme instability.Set up an end-point radiometric method for activity measurements.Cloned and subcloned proteins, designed and obtained cloning and expression constructs.Contributed to projects design, planned and performed experiments, interpreted results, wrote papers, designed and prepared all figures.Communicated orally, in writing, and at diverse levels in international environment on the daily basis.Supervised two undergraduate students working on subprojects of my own research.Taught or supervised lab classes (8-22 undergraduates) in Biochemistry and Biochemical Methodology, over 1000 h in total.Presented materials covering the relevant chemical/biochemical topics, designed mini projects, experiments, tests, graded reports, instructed in scientific writing, lab safety, waste disposal.Experienced with dyslectic students.

Obtained barnase mutants by site-directed mutagenesis and made several expression constructs.Expressed and purified cysteine-containing barnase mutants.Contributed to study of expression of bioengineered antibody-conjugated proteins.Wrote a summary of bioconjugation methods (chemical and bioengineering approaches).