Project: : “Genome Engineering to Sustain Crop Improvement (GETSCI)” with focus on developing and applying improved tools to suppress the late flower behavior of high yielding tropical maize inbreds, allowing them to be used immediately in any maize breeding program, worldwide.

Planning and execution of research experiments in the areas of in vitro diagnosis and development of molecules and processes used to treat diseases; Data analysis and discussion; Preparation of technical/scientific reports; Responsible for requesting and controlling stock of reagents and laboratory materials through the Senior ERP system; Preparation and standardization of solutions.Main activities: general techniques of molecular biology and biochemical assays, including extraction and… Show more Planning and execution of research experiments in the areas of in vitro diagnosis and development of molecules and processes used to treat diseases; Data analysis and discussion; Preparation of technical/scientific reports; Responsible for requesting and controlling stock of reagents and laboratory materials through the Senior ERP system; Preparation and standardization of solutions.Main activities: general techniques of molecular biology and biochemical assays, including extraction and quantification of nucleic acids; PCR; agarose gel electrophoresis; plasmid purification; isolation, purification assays (liquid chromatography system, Akta) and protein characterization (SDS-PAGE polyacrylamide gel electrophoresis, Western Blot, ELISA).Basic training for the use of bioreactors: Techfors (Infors HT), BioFlo 320 (Eppendorf) and DasBox mini reactor system (Epperndorf)Main Results: structuring and organizing the laboratory, improving time management, optimizing protein purification processes. Show less

Leadership and coordination of the COVID-19 sample receiving, screening and processing team in the Clinical Analysis LaboratoryResult: training of new employees in the functions to be developed; certificate of excellence for virtuosity and dedication to the laboratory

Main activities: Molecular biology and biotechnology techniquesResults: Laboratory organization, improved work environment and increased productivity with implementation of the 5S program

Private class for undergraduate students Discipline: Biochemistry

Project: “Construction of an excretory ammonium strain of Azospirillum brasilense containing a point mutation in the glnA gene” and “Characterization of glutamine synthetase of excretory ammonium strain HM053 from Azospirillum brasilense" Main activities: construction of an excretory ammonium bacterial strainn, with the objective of being used as a plant inoculant. Planning and carrying out experiments; general molecular biology techniques and biochemical assays, including isolation and… Show more Project: “Construction of an excretory ammonium strain of Azospirillum brasilense containing a point mutation in the glnA gene” and “Characterization of glutamine synthetase of excretory ammonium strain HM053 from Azospirillum brasilense" Main activities: construction of an excretory ammonium bacterial strainn, with the objective of being used as a plant inoculant. Planning and carrying out experiments; general molecular biology techniques and biochemical assays, including isolation and quantification of nucleic acids; PCR; agarose gel electrophoresis; culture and transformation of bacteria; bacterial conjugation, plasmid-based bacterial gene cloning; restriction enzyme analysis of plasmids and genomic DNA; plasmid purification; analysis of DNA sequence data; nitrogenase assay; ammonium and protein dosage; isolation, purification assays (liquid column chromatography), identification and characterization of proteins (SDS-PAGE acrylamide gel electrophoresis, Western Blot, ELISA, Maldi-TOF mass spectrometry); PyMol analysis; etc. Results: improvement in adaptability and flexibility in the face of adverse and unimagined situations; autonomy in decision making and personal management Show less

Project: “Anti-inflammatory properties of curcumin on the NLRP3 inflammasome/IL-1 b secretion pathway”Main activities: Biochemical techniques including mammalian cell culture, sodium dodecyl sulfate polyacrylamide gel analysis (SDS-PAGE), Western Blot, ELISA and staining techniques such as DCF and MitoSOX.Results: improved critical analysis and intellectual maturity; presentation of work in international congress and contribution as co-author of scientific articles.

Main activities: activities in the fields of molecular biology of plants and biotechnology as: isolation of DNA and RNA; quantification of nucleic acids; PCR; agarose gel electrophoresis; rapid amplification of 5 'and 3' ends of cDNA (RACE); culture and transformation of bacteria; cloning of plant genes based on the plasmid; purification of the plasmid; restriction enzyme analysis of plasmids and plant genomic DNA; data analysis, crude DNA sequence and similarity gene database searches; plant… Show more Main activities: activities in the fields of molecular biology of plants and biotechnology as: isolation of DNA and RNA; quantification of nucleic acids; PCR; agarose gel electrophoresis; rapid amplification of 5 'and 3' ends of cDNA (RACE); culture and transformation of bacteria; cloning of plant genes based on the plasmid; purification of the plasmid; restriction enzyme analysis of plasmids and plant genomic DNA; data analysis, crude DNA sequence and similarity gene database searches; plant tissue culture techniques; and plant transformation techniques such as Agrobacterium and gene-gun.Results: learning new molecular biology techniques and improving already known techniques Show less

Project: "Evaluation in vitro of antioxidant activities and antimicrobial of black tea extracts hydroalcoholic (Camellia sinensis) and shiitake mushrooms (Lentinula edodes) and shimeji (Pleurotus ostreatus) " Main activities: antioxidant assays as: conventional extraction of bioactive compounds, quantification of total phenolic compounds, FolinCiocalteau method, agar diffusion test with well drilling and Minimum Inhibitory Concentration (MIC)Results: improvement in the ability to search… Show more Project: "Evaluation in vitro of antioxidant activities and antimicrobial of black tea extracts hydroalcoholic (Camellia sinensis) and shiitake mushrooms (Lentinula edodes) and shimeji (Pleurotus ostreatus) " Main activities: antioxidant assays as: conventional extraction of bioactive compounds, quantification of total phenolic compounds, FolinCiocalteau method, agar diffusion test with well drilling and Minimum Inhibitory Concentration (MIC)Results: improvement in the ability to search for bibliographic references, search for different sources of information and exchange ideas with other students and teachers Show less

Main activities: cultivation of the microcrustacean Daphina magna; sensitivity test with the microcrustacean.

Production and organization of social and corporate eventsResults: discernment and agility to face difficulties and seek solutions to problems, skills to multitask, work in teams, communicate with people and improve proactivity