Feroza Iffat Email & Phone Number

Conroe, Tx, Us

Conroe, Tx, Us

Durham, North Carolina, Us

• Developed complement dependent cytotoxicity (CDC) assay for different antibodies. Maintained several cell lines including HER2-expressing Raji cells and Daudi cells.• Reviewed several new client proposals, researched to make a good PD plan for the client including process optimization via closed system and automation. Also play role in identifying factors effecting the logistics including timeline and cost of the project. Actively participated in experimental designs. • Identified several new equipment, organized onsite/virtual demos, and evaluate the equipment for potential projects.

Durham, North Carolina, Us

Promoted to Process Development Scientist II• Performed mTCR manufacturing process including Sleeping beauty integration via electroporation- based transfection strategy. Method involved Sepax, Nucleofector, CliniMACS Plus and Gatherex. Also performed related analytical testing to support phenotypic and functional characterization including moDCs generation for co-culture, flowcytometry via CytoFLEX, potency assay, ELISA and VCN. Authored ATMs, MBRs and summary reports. PD lead for tech transfer.• Performed CAR-T cell product manufacturing process using the CliniMACS Prodigy TCT program involving LVV transduction. Also performed analytical testing to support phenotypic characterization including flowcytometry via Cytoflex. Authored ATMs, MBRs and summary reports. PD lead for tech transfer.• Supported antigen specific T-cells using overlapping peptide pools as antigen source for growing large numbers of antigen specific T-cells. Method involved Elutra Cell Separation System, LOVO cell washing device and G-REXs.

Houston, Texas, Us

• Designed, conducted, analyzed, and reported results to support the company’s product development and characterization of new products.• Collaborated with research teams for the development, troubleshooting, and optimization of new cryopreservation method development.• Successfully evaluated and implemented improved method of NK cells enrichment by applying the Miltenyi Biotec LP-3-56 system. • Identified GMP compliant cryoprotectant and cryopreservation method for NK cells with excellent cell recovery and viability post-thaw.• Carry out experimental designs for NK-cells in G-REX system stimulation and expansion. • Performed phenotypic analysis using NovoCyte, Gallios Flow Cytometer and Novo Express and Kaluza Analysis Software.• Completed testing of implementation of closed system for manufacturing of GoCAR-T® (BPX-601) in CliniMACS Prodigy® T Cell Transduction (TCT) system.

Houston, Texas, Us

• Performed process modification study for Rivo-cel (BPX-501) to improve the number of generation CAR-T cells to support GMP manufacturing of higher doses for patient treatment. • Evaluated and implemented G-REX platform for the BPX-802 allogenic product.• Independently designed and executed cytokines and media replenishment schedule, and testing per DOE for BPX-603 dual-switch GoCAR-T. • Executed DOE for activation and proliferation of CAR cells for the autologous GoCAR-T platform.• Successfully tested the multiple versions of CliniMACS Prodigy ficoll program to improve recoveries and RBCs contamination.• Identified GMP-grade and performed testing of the secondary sourcing reagents for clinical manufacturing.• Performed flow cytometric analysis using Gallios Flow Cytometer and Kaluza Analysis Software.• Independently performed data analysis using Microsoft Excel and GraphPad Prism.• Performed apoptosis and potency assays (ELISA). • Evaluated new equipment including Xuri Cell Expansion System W25 and Sefia Cell Processing System for CAR-T cell GMP manufacturing. • Wrote protocols, reports, test-methods, SOPs and Batch Records (BRs) and maintained to GMP compliance.

Developed cryopreserved T-cell immunotherapy as a final product: Testing different point of irradiation for Cryopreserved T-Cell ImmunotherapyMethod evaluation for thawing cryopreserved dose equivalent vialEvaluation of alternative dose vials for cryo storage and gamma irradiationHandling temperature during T-Cell Immunotherapy cryopreservationProduct characterization – ELISA/ Luminex (Magpix) based multiplex assay and data analysis via Milliplex analysts, Specificity analysis assay (EliSpot), Phenotyping using Flow cytometry (Attune)T-cell expansion – Antigen specific cell-lines in closed system (culture bags), T-cell blast (flasks), Bead expansion, APC (antigen presenting cells) preparation using Gamma cell 3000 Elan irradiator, CD25, CD235 depletionProduct purification – Density gradient separation, Magnetic bead depletionDocumentation/data analysis – DOEs, SOPs, Technical Report writings, Graph Pad (Prism), Internal Audits, Data interpretation and process optimization

Assist doctor in routine clinical procedures Demonstrate strong phone skills, maintaining excellent patient relations.Supervise staff members in absence of office manager.Coordinate and serve as clinical resource person, providing appropriate office management supports Maintain and monitor professional competency growth of staff and self.

A.T.C. Biotechnology (Texas State Skills Board Certified Program), Biotechnology

Medical Assistant, Medical Assistant

Master’S Degree, Physiology (Human)

Bachelor’S Degree, Physiology (Human)