Flora Bunga, Phd Email & Phone Number

London & Shannon

Galway, County Galway, Ireland

Working on the method development of complex large molecules.• Developing phosphorylation methods, PCR-based reactions.• Quantification of protein impurities using the ELISA technique.• Developing the HPLC-based separation methods for the large biological molecules

Brest, Brittany, France

From blood isolation of PBMC and characterized biomarkers of Sjögren's syndrome.• CD4+, CD8+ and T cells were purified from peripheral blood using a magnetic separation technique on a CliniMACs Plus instrument.• The percentage of CD3+, CD4+, CD8+ and CD19+ cells in the selected population was determined by flow cytometry as quality control metrics

Nantes

• Culture of primary and cell lines to produce cell therapy drug for a skin graft (Cicafast).• Cells were transducted using viral vectors.• Performed extractions of RNA using a Triazol kit to identify biomarkers of the transduction by RT-qPCR.• Identity of the cells was confirmed by biomarkers using flow cytometry and immunofluorescence techniques.• Potency assay and ELISA were performed for cell bioassay purposes.• Developed and maintained detailed records of experiments.• Analyzed data, wrote SOPs, and tracked non-conformances.• Ensured that all work complies with GCP, GLP and all relevant SOPs.• Managed time effectively to meet clinical demands and deadlines.

• Quality control on anti-allergen patches: extraction of the protein API.• Identification of API by different techniques such as Bradford, SDS-PAGE tests.• Performed quantification of API using ELISA.• Worked in GMP regulations, completed LIMS, tracked non-conformance.

Nivelles, Wallonia, Belgium

Culture primaire, passages, entretien des lignées, transfection, test de cytotoxicitéAnalyses biochimiques et immunologiques: TCID50, ELISA, Western Blot, SDS-PAGE, PCR, Techniques de production des protéines recombinantes, extraction, purification et analyse d'ADN

Belgium

Paris Area, France

Bishops Waltham, Hampshire, Uk

Master 2 Biothérapies Et Médicaments De Thérapie Innovante, Biotechnologie

Activities and Societies: Thérapie cellulaire et génique. Contrôle qualité des MTI, BPF, Assurance qualité

Phd Student, Medicinal Chemistry At Pharmacy And Pharmacology Department

Master'S Degree, Organic Chemistry, Erasmus Program

Master'S Degree, Chimie Organique Et Analytique

Licence De Chimie, Chemistry

Dut Chimie, Organic Chemistry

Baccaleauréat Stl Chimie

Education record