Florian Noack Email & Phone Number

Penzberg, By, De

Penzberg, Bavaria, Germany

Munich Area, Germany

Identification and validation of gene regulatory elements for the next generation of gene therapies. During my Postdoc, I developed a novel multiomic next generation sequencing approach which allow to measure simultaneously gene expression, chromatin accessibility, DNA methylation and 3D chromatin structure. Together with commercial (single cell-) multiomic methods and an integrative data analysis strategy, I was able to identify gene regulatory elements during mouse and human brain development. Subsequently, I validated these genetic elements for the first time in vivo using a newly established massive parallel reporter (MPRA) assay and currently I investigate their potential to drive cell-type specific gene expression for gene therapeutical applications in combination with AAV.Key techniques: • (sc)RNA-seq, (sc)ATAC-seq, CITE-seq, SPRITE-seq, sci-RNA-seq, Massive Parallel Reporter Assays (MPRA), WGBS, NOMe-seq, EM-seq, (Methyl-)HiC, (h)MeDIP-seq, ChIP-seq, CUT&RUN• development of a novel multiomic next generation sequencing method• bioinformatic analysis (cell-ranger, deepTools, Seurat, Signac, ArchR, Juicer,costume made R-scripts, bash scripts etc.)• surgery and dissection of mice, immuno-FACS• mouse embryonic stem cell culture, cell line engineering

Dresden Area, Germany

Assessment and site-specific manipulation of DNA (hydroxy-)methylation during mouse corticogenesis.During my PhD I mapped the genome-wide patterns of DNA (hydroxy-)methylation during cortical development and developed new tools to site-specifically manipulate them in vivo using a modified CRISPR/Cas9 system. Key techniques:• surgery and dissection of mice• (hydroxy-)methylated DNA immunoprecipitation (hMeDIP-seq), ChIP-seq, ATAC-seq,RNA-seq• bioinformatic analysis (Bismark, deepTools, Diffbind, Homer, costume made Rscripts,bash scripts etc.)• development of epigenome editing tools (CRISPR-dCas9-Tet1 system)

Dresden Area, Germany

I successfully completed my diploma thesis with the title “Analysis of gene expression and epigenetic regulation in the dentate gyrus of mice living in an enriched environment” in the laboratory of Prof. Dr. Gerd Kempermann. During my project, I compared the expression of various neurogenesis related genes in the dentate gyrus of mice housed in standard or environmental enrichment cages. Based on these data, I chose candidate genes to analyze the DNA methylation pattern of their promotor regions. For this purpose, I introduced a method called bisulfite sequencing which allows mapping methylated cytosine of a specific region with a single base resolution.Key techniques:• surgery and dissection of mice• behavioural test (morris water maze)• bisulfite amplicon sequencing• establishment of real time RT PCR assays

Perth, Australia

I completed an internship in the laboratory of Prof. Dr. Archa Fox who focuses on Paraspeckles, a RNA-protein complex of the cell nucleus. In order to identify interacting sites of a known paraspeckle-associated protein, I conducted a co-localization study using different fragments of this protein.

Penzberg, Germany

The aim of my long-term internship in the lab of Dr. Martin Schäfer was to establish and compare different IgG purification methods. In order to test these methods, I developed and established a high throughput (384 well) ELISA based test assay using an automated system.Key techniques:• ELISA, immunoblot• optimization of an automated system (Biomek 3000)• nAB Assay and mammalian cell culture

Phd, Molecular Biology, Summa Cum Laude (1.0)

Diploma Degree, Biology (Specialization In Genetic, Immunology, Zoology), 1.7 (Very Good)

Activities and Societies: Student research assistant in various labs (Chair of Genetics; Chair of Plant Cell and Molecular Biology; Chair.