Specializing in Cellular and Gene Therapies (CGT) using CAR (Chimeric Antigen Receptor) engineered cells, I am passionate about enhancing cancer treatment accessibility and efficacy. My focus is on bridging the gap between innovative research, manufacturing, and clinical trials, prioritizing patient safety, quality, and successful outcomes.With over 1️⃣4️⃣ years of experience in bio/chem engineering and clinical research, my work is characterized by a commitment to quality and meticulous data analysis, ensuring all documentation meets regulatory standards. Join me on this journey of scientific discovery and excellence.𝗕𝗶𝗼𝗮𝗻𝗮𝗹𝘆𝘁𝗶𝗰𝗮𝗹 𝗔𝘀𝘀𝗮𝘆 𝗗𝗲𝘀𝗶𝗴𝗻 𝗘𝘅𝗽𝗲𝗿𝗶𝗲𝗻𝗰𝗲I have extensive experience working in both bacterial and mammalian cells. I enjoy designing and developing bioassays, including Flow cytometry, ddPCR/qPCR, ELISA, FPLC, and Western blot. I led the Analytical Development department, overseeing method transfers and ensuring compliance with FDA and regulatory standards. My responsibilities extended beyond the lab to include business development, strategic planning, and client onboarding. My technical expertise consistently delivers high-quality results.𝗘𝗱𝘂𝗰𝗮𝘁𝗶𝗼𝗻𝗮𝗹 𝗕𝗮𝗰𝗸𝗴𝗿𝗼𝘂𝗻𝗱 𝗮𝗻𝗱 𝗘𝘅𝗽𝗲𝗿𝗶𝗲𝗻𝗰𝗲My PhD equipped me with the skills to embrace and adapt to new ideas, train myself, and approach critical problems from scientific and general perspectives. While extensively investigating protein structure-function relationships and enzyme kinetics, my takeaway experience was how to apply it in clinical fields. This enabled me to contribute to projects differently, leveraging my protein expertise to solve clinical hurdles. During my postdoctoral research at the NIH, I focused on corticosteroid-independent protein mimetics for treating refractory asthma. This diverse background has deepened my understanding of both academic research and its practical applications in the industry𝗟𝗲𝗮𝗱𝗲𝗿𝘀𝗵𝗶𝗽 𝗦𝗸𝗶𝗹𝗹𝘀I have demonstrated strong leadership skills in managing multiple projects and leading cross-functional teams. I ensure successful method transfers and project completions. My PMP certification enhances my project management capabilities. My leadership emphasizes transparent communication, strategic planning, and fostering a collaborative team environment.Let's connect and explore how we can collaborate to push the boundaries of biotechnology and cancer treatment.📧 𝘀𝗵𝗮𝗵𝗶𝗱.𝘀𝗵𝗮𝗵𝗶𝗱𝟱𝟱𝗸@𝗴𝗺𝗮𝗶𝗹.𝗰𝗼𝗺
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Development Scientist (Team Lead)Biocentriq Feb 2023 - PresentNewark, New Jersey, Us• Creating an Immuno-Oncology platform to advance CAR-T, TCR, and NK cell-based immunotherapy• Optimized large-scale NK cell expansion and transfection using G-Rex, Amb15, and STR50 • Developing and implementing cell and gene therapy drug products, AAV, Lentivirus Vector, etc.• Design, develop, and execute Multicolor Flow Cytometry for immune cell assays or characterization (SME: CytoFLEX, BD FACSMelody, BD FACSLyric, proficient software: FlowJo, BD FACSDiva, BD FACSChorus, tSNE, UMAP FlowSOM, PhenoGraph, SPADE, Citrus, Kaluza, and CytExpert)• Establishing advanced bioassay, cytotoxicity, potency, and ADC assays following FDA guidelines• Execute numerous qPCR/ddPCR assays for RCL, VSVG, and VCN analysis• Purified large scale AAV5 using STR50 and quantified and characterized using AKTA Avant• Conduct comprehensive Flow Cytometry and ddPCR/qPCR, Western Blotting training sessions for internal team members and QC• Drafting SOPs, Qualification reports, and Method transfer protocols in accordance with GLP/GMP guidelines to meet regulatory standards (ICH, Eur. Ph., USP, FDA, and JP guidelines).• Effectively team up with cross-functional departments (PD, QC, MSAT) and aid in risk management, handling deviations, and CAPAs to sustain compliance with cGMP regulations -
Analytical Development Scientist (Entire Site Closed)Catalent Pharma Solutions Jul 2022 - Feb 2023Somerset, Nj, Us• Planned and designed clinical experiments for new drugs• Executed and performed Dendritic-based research as a Cancer Vaccine• Formulated, consolidated, and expanded studies involving in vitro human immune cell culture assays• Executed multicolor flow cytometry to identify and treat cancer cells • Performed ELISpot, ELISA, and cytokine staining to analyze inflammatory markers on the patient's blood• Collaborated across functions with other teams on immunological workflows• Mononuclear cell fraction from apheresis product using NeatCell C-Pro software with Sepax C-Pro instrument• Served technical resources for analytical method development, tech transfer, data interpretation troubleshooting• Prepared and reviewed QC SOPs, protocols, and reports related to product release, stability, and method validation.• Collaborated across functions with other teams on immunological workflows -
Postdoctoral ResearcherNational Heart, Lung, And Blood Institute Mar 2021 - Jul 2022Bethesda, Maryland, Us• Independently designed and executed experiments to define and test hypotheses for Apolipoprotein L2 on unfolded protein response and apoptosis using mammalian cell line and human whole blood/elutriated monocyte cells (CD14+).• Multicolor Flow Cytometry: Characterized immune response in Lung inflammation; especially monocyte, T-cell, and neutrophil activation. Intracellular staining for pro/anti-inflammatory cytokines. CD14+ cells sorting Flow cytometry data analysis using the FlowJo software package.• Cell culture maintenance (THP1, SAEC, CD14+), transfections, overexpression & gene knockdown using siRNA.• Protein characterization including HPLC, ELISA, SDS-PAGE, native PAGE, western blot, and endotoxin assay. • Implemented clinical methods for developing corticosteroid-independent drugs for Asthma. • Incorporated cell-based assays to support the identification, optimization, and characterization of novel mimetics and their targets.• Responsible for more complex assignments and more than 1 project.• Participate in self-development activities and several professional pieces of training arranged by NIH. -
Ph.D. Student And Graduate Research AssistantUniversity Of Wisconsin-Milwaukee Aug 2015 - Dec 2020Milwaukee, Wi, Us• Developed a new mechanism of cytochrome c nitrite reductase (ccNiR) enzyme ccNiR in anaerobic conditions using glovebox, stopped-flow, time-resolved, rapid freeze-quench spectroscopy • Trapped reaction intermediate for the first time and quantified using kinetic simulation• Molecular cloning including PCR, DNA digestion, ligation, and side-directed mutation of active site (H257QccNiR, R103QccNiR) gene and expressed into Shewanella oneidensis bacteria• Improved chromatographic techniques for high-yield protein purification with FPLC including affinity, ion exchange, HIC, Size-Exclusion, etc.• Designed UNICORN™ 7.0 software programs to purify large scale protein automatically• Effectively utilized standard laboratory equipment including hand pipettes, balances, pH meters, plate readers • Introduced lab for large-scale culture growth (50 L) technique for purification of bulk protein • Published two scientific articles and 2 manuscripts are in preparation• Findings led to getting two successive NSF funding and being shortlisted for Linac Coherent Light Source Facility at Stanford University for time-resolved crystallography -
Graduate Teaching AssistantUniversity Of Wisconsin-Milwaukee Aug 2015 - Dec 2020Milwaukee, Wi, Us• Performed both lecture and laboratory classes including Biochemistry, Chemistry for Engineer, etc.• Taught more than 120 students each semester, graded quizzes, and exams for a total of 15 semesters• Performed online classes, recorded video laboratory classes during Covid 19 emergency • Introduced new teaching methods and grading strategy
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Quality Control OfficerAci Limited Jun 2013 - Jun 2014Dhaka, Bd• Led to improve efficiency & method validation of drugs • Skilled in multiple analytical techniques (HPLC, GC, TLC, etc.) for drug optimization• Achieved outstanding performance in industrial biosafety training • Designed and developed experimental SOP, GLP -
Elective Research FellowIcddr,B Aug 2011 - Dec 2012Dhaka , Dhaka, BdInvestigated the role of vitamin B 12 in nutritional anemia by measuring immunomarker (H1N1 IgA, IgG, AGP, Hb)• Trained for antibody for lymphocyte supernatant (ALS) technique• Trained for separation of PBMC and PMN• Trained for different serological techniques; PCR, RT-PCR, ELISA
Fnu Shahid, Ph.D. Pmp Education Details
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University Of Wisconsin-MilwaukeeProtein Biochemistry And Enzyme Kinetics -
University Of DhakaBiochemistry And Molecular Biology
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University Of DhakaBiochemistry And Molecular Biology
Frequently Asked Questions about Fnu Shahid, Ph.D. Pmp
What company does Fnu Shahid, Ph.D. Pmp work for?
Fnu Shahid, Ph.D. Pmp works for Biocentriq
What is Fnu Shahid, Ph.D. Pmp's role at the current company?
Fnu Shahid, Ph.D. Pmp's current role is Cell and Gene Therapy.
What schools did Fnu Shahid, Ph.D. Pmp attend?
Fnu Shahid, Ph.D. Pmp attended University Of Wisconsin-Milwaukee, University Of Dhaka, University Of Dhaka.
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