Fernando Ortiz Scym(Ascp) Email & Phone Number

Ottawa, Canada Area

• Primary consultant for spectral/flow cytometry, cell sorting and antibody panel design.• Develop, plan and deliver training for personnel and clients for spectral/flow cytometry and cell sorting techniques.• Responsible for financial reporting, including billing, budgeting, grant applications, procurement.• Generation and implementation of SOPs and quality assurance plans.• Management of all services, including maintenance and repair of flow cytometers and cell sorters: MoFlo XDP, SONY MA900, BD LSR Fortessa, Cytek Aurora.• Management of flow cytometry software licenses and additional training (FlowJo, FCS Express)• Trained on Zeiss LSM900 with AiryScan and Leica SP8 confocal microscopes• Custodian of Fluidigm C1 and BioMark HD single-cell analysis equipment.• Workflow consultation and sample preparation for Amnis ImageStream MK II imaging flow cytometry operators.• Certified deputy in-house operator of Helios mass cytometer.

Toronto Y Alrededores, Canadá

• Enumeration of CD34+ cells in cord blood and breast cancer samples using BD FACSCalibur• Assessment of cell surface markers associated with minimal residual disease using BD FACSCanto II• Evaluation of immunological activation profiles associated with Treatment-free Remission Accomplished with Dasatinib in patients with CML using BD FACSCanto II• Isolation of CD8+ and NK cell populations for gene expression profiling.• Development of 5-C, 6-C and 7-C assays for Paroxysmal Nocturnal Hemoglobinuria (PNH) WBC detection and PNH RBC enumeration for BD FACSCanto II and Navios.• Generate and maintain standard operating procedures (SOPs)• Generate and implement a quality assurance plan for flow cytometry assays and all dating to ensure that all assays are reproducible and high quality• Responsible for archiving and managing scientific and routine patient data, and generating quality control reports

Toronto Y Alrededores, Canadá

• Study of mimic glycosphingolipids inhibition of HIV infection and cytotoxicity• Maintain and culture fibroblast cell lines expressing CCR5 and CXCR4• Develop and execute ELISA and Western blot assay to test glycosphingolipids inhibition of Gp120

Toronto Y Alrededores, Canadá

• Develop, plan, and deliver lessons on linguistics and practical language use to adults• Motivate students and create positive classroom setting (as evaluated by student surveys)

Sevilla Y Alrededores, España

ARTANA Research Group studies the internal structures of living forms, focusing on human anatomy.• Developed studies of vegetal and animal anatomy, and mounting and cleaning protocols for animal bone structures• Created and executed new protocol for tissue and cell culturing (Projects: COLONIES and LUMIX) • Implemented partnerships between municipalities and companies with the ARTANA Research Group

Sevilla Y Alrededores, España

Planned, developed and delivered lessons for second year undergraduate students:- General morphology for the applied anatomy.- Mounting and cleaning protocols for animal bone structures.- Determination of vascular plants of Western Andalucía.- Mounting of creative textures applied to the human anatomy.

Sevilla Y Alrededores, España

Conducted Environmental feasibility and sustainability studies, as well as urban and management planning for the municipalities of Llanos del Caudillo, Picón, Arenas de San Juan, Alcolea de Calatrava, Argamasilla de Alba (Ciudad Real). Carried out mapping studies, environmental impact analysis, spatial planning, pluviometry, irradiation, population studies.

Madrid Y Alrededores, España

• Developed study of the HIV-1 co-receptor usage in non-B genetic forms from plasma samples of HIV positive patients from Spain in a biosafety level 2 laboratory • Completed amplification of partial pol Protease/Reverse Transcriptase and V3 sequence of HIV-1 • Realized RT-PCR, PCR, gel electrophoresis, purification and sequencing • Processed sequence of partial HIV-1 tropism/co-receptor usage by bioinformatics programs • Evaluated phylogenetic studies of the V3 sequence of HIV-1. Analyzed and corrected partial HIV-1 sequences • Implemented new laboratory staff orientation, reducing training time in 1 month • Piloted a new product testing process, saving 40 % in annual product consumption

Industrial Pharmaceutical Technology, Pharmaceutical Sciences

Certificate In Life Science Enterprise

Master'S Degree, Virology

- Specialty in Veterinary Virology - Specialty in Human Virology Final Masters Thesis: The HIV co-receptor usage in non-B genetic forms.

Master'S Degree, Biology/Biological Sciences, General

Experimental techniques applied for the following subjets: - Biochemistry - Botany - Plant and Animal cytology and histology - Ecology.

Bachelor'S Degree, Biology/Biological Sciences, General

Main fields of study for the qualification: - Biochemistry - Botany - Plant and Animal cytology and histology - Ecology - Plant and animal.

High School Diploma, Health Sciences