Study of the impact of integrated HBV viral sequences on circulating HBV RNAs in the chronic infection by next generation sequencing (NGS), using the sequencer Miseq (Illumina platform), study of hotspots, chromosomal rearrangements, mutations at the gene level, characterization of circulating HBV RNAs by oxford nanopore sequencing using the sequencer minION (ONT), optimization of protocols, interpretation and data analysis, quantification of nucleic acids by digital droplet PCR (ddPCR) and quantitative PCR (qPCR), generation of clonal cell lines and cell cultures (liver cells), use of Cobas instrument (ROCHE)This is a project included in the "Recherche hospital-Universitaire" (RHU) in collaboration with ROCHESee the link below: https://www.cirb-rna.fr/

Study of the integration of the hepatitis B virus (HBV) within the human genome by next generation sequencing (NGS), using the sequencer Miseq (Illumina platform), study of hotspots, chromosomal rearrangements, mutations at the gene level, sequencing of nucleotide sequences by pyrosequencing and Sanger method, ELISA test, DNA/RNA extraction, purification and quantization of nucleic acids (Qubit), reverse transcription, spectrophotometric reading (NanoDrop), RT-PCR, southern blot, optical microscopy analysis (bright field, Giemsa method), cellular cultures, microarrays, nucleotide and amino acid alignments (bioedit), cell transfection techniques, bioinformatics analysis, use of Nanopore technology

Study of the occult infection of the hepatitis B virus (HBV) using the digital droplet PCR (ddPCR), extraction of nucleic acids (DNA, RNA, cccDNA) from liver biopsies, use of the Quantasoft software for the analysis of data obtained performing the ddPCR technique, extraction of nucleic acids from supernatant and cell portion of biological samples, cell cultures (liver cells), generation of monoclonal cell lines, use of GraphPad Prism software

Development, optimization and validation of immunoassays for the evaluation of antigenicity and for the release of vaccine batches (phase I, II, III) by ELISA test, direct and indirect method, sandwich ELISA, mAbs screening, respecting the Good Manufacturing Practices (GMP) and working according to SOAP

Industrial bacterial fermentation, preparation and use of bioreactors (2L, 20L), microbial staining (DAPI, Gram staining), use of inverted fluorescence microscope (AxioObserver Z1), use of AxioVision software, immunofluorescence techniques, use of monoclonal antibodies, nucleic acids extraction and quantification of (DNA/RNA) by qubit and bioanalyzer, pcr, characterization of different bacterial strains, spectrophotometric analysis (NanoDrop), CFU, chemical analyzer

During my Erasmus placement period I investigated the effects of subchronic exposure of zebrafish to ibuprofen and to other toxic substances, using selected oxidative stress parameters as a target.