MeliBio strives to produce plant-based honey without harming bees. Creating bee-free honey alternatives allows these keystone pollinators to not be exploited and encourages diversity amongst native bee/pollinator species.- Resident protein chemist and Bio-Analytics expert. Acted as fermentation/DSP specialist.- Responsible for developing, validating and creating pipelines to support protein purification and characterization. - Purified recombinant honeybee enzymes via batch and column affinity chromatography from bacteria and yeast. Optimized purification process to increase yield and reduce time to purify protein by 50 %. - Created infrastructure surrounding data & SOP management, LIMS, lab management and inventory. - Screened enzyme targets from Strain Engineering Team to drive fermentation, stability and shelf-life studies. This involved establishing a high-throughput strain screening platform to provide quantitative data on strain performance. - Designed and carried out preliminary product development work, including stability & shelf-life studies.- Managed relationship with fermentation CRO, advising on bioreactor experiments, DSP and Analytics experiments, which lead to a 14x increase in target protein titer. This involved in depth knowledge of strains, target proteins and in-process steps, including TFF, microfiltration, ultrafiltration and diafiltration.

Perfect Day produces dairy proteins using precision fermentation. Our approach generated whey proteins with identical structure and food functionality as traditional dairy from cows with a fraction of the environmental footprint.- Led the Biochemistry effort at Perfect Day Berkeley. I managed a dynamic team to bring reliable, accurate assays to R&D via robust method development and validation avenues. - Expertise in protein purification and characterization via FPLC (SEC, IEX, affinity, HIC, multi-modal), PAGE (SDS-PAGE, nPAGE, IEF, 2D-PAGE, Western blotting) and 96 well plate assays to probe structural/functional relationships (absorbance, fluorescence, colorimetric).- Developed and routinely performed a diverse range of biophysical and biochemical techniques to probe protein integrity & stability, behavior and identity including proteomics, DSC, differential scanning fluorometry (DSF), dynamic light scattering (DLS) and phase assisted light scattering (PALS).- The Biochemistry Team explored a broad range of sample matrices to test hypotheses concerning protein behavior and functionality. We served and collaborated with diverse teams, including Strain Engineering, Screening, Fermentation, DSP, Food Discovery, Commercial and QA/QC.- Expanded managerial and non-scientific skillset, including budget generation & planning, Capex requests and ROIs, promotions & advocation, project planning and managing, goal planning and succession/growth planning.

- Developed expertise in high throughput method development & validation of protein/biomolecule interaction assays. To combat sensory issues present in final products a kinetic enzymatic assay was established to assess quality risk across a range of biomolecules. This work identified strains and process conditions that presented a reduced risk of sensory failure and allowed data-driven progress towards a higher quality product for food applications.- Demonstrated capability to technically lead and manage projects encompassing scientists with diverse skill sets when new protein targets were onboarded. Drove protein characterization to gain structural and behavioral results via FPLC, PAGE, HPLC, DSC, IEF and ANS hydrophobicity assay. Consolidated a wealth of data into concise, detailed reports and reported results to key stakeholders to inform the feasibility of pursuing protein targets for further development and food applications.- Successfully collaborated with Food scientists to develop peptide SEC and peptide PAGE methods to quantify the degree of hydrolyzation in powder samples based on molecular weight ranges of interest. This method identified optimal hydrolyzation conditions for the Food Team to generate products that met specifications required by external customers. - Identified a technical capability gap within the team regarding the lack of a high throughput protein thermostability assay to inform how strain, fermentation and process changes influence stability. Advocated and justified the purchase of instrumentation for real-time thermostability measurements, and maintained a fruitful relationship with the vendor to negotiate a 20 % discount.

- Worked on a multi-team collaboration to solve quality issues in final products and resulted in a published patent. This involved deep understanding of protein and small molecule interactions, enzymology and protein separation techniques including HIC and SEC. - Designed and generated several assays that required fundamental knowledge of protein structure and predictive solution behavior under different solvent conditions, including Ellman’s assay to probe free thiols, native PAGE and SEC. These assays were routinely utilized to inform stakeholders whether protein integrity is maintained following fermentation and in-process changes. - Advocated and justified purchase of an FPLC system and negotiated a 30 % discount on the system with the vendor. The subsequent work carried out on the FPLC allowed the instrument to reimburse itself within 6 months.

- Engineered highly robust, 2D self-organizing S-layer proteins for the simultaneous and controlled deposition of multiple types of nanoparticles. Modified two protein-conjugation systems, coupled orthogonally to allow the creation of bimetallic arrays with plasmonic properties. - Characterized proteinaceous hybrid materials using fluorescence microscopy, IRM, SEM/STEM/HR-TEM and SAXS.- Primary researcher and communicator in a multidisciplinary team of biologists, chemists and physicists to analyze plasmonics and visualize hybrid materials. - Presented research to a range of audiences, from general public to academics, at lab tours, international conferences and through peer-reviewed papers. - Developed and implemented protocols for protein engineering and soft sample imaging.- Optimized experiments for DNA manipulation as well as protein expression and isolation.

- Instructed and trained student in skills typically employed in a biochemistry/protein analysis laboratory. - Developed a project for student and directly supervised her progress and technical skill development.

- Embellished synthetic ring-forming proteins to fabricate novel tubule and cage nanostructures.- Used a range of biophysical techniques including SEC, MALLS, TEM and SAXS to characterize proteins.- Probed RNA-binding capabilities of engineered proteins with DSF, ITC.- Developed expertise in protein crystallization and a range of crystal optimization avenues, leading to the first crystal structure of a synthetic tetrameric Lsm ring composed of four engineered polyproteins.- Recipient of the Australian Postgraduate Award (2012 - 2015).- Presented results at a number of international conferences and received student poster prizes in consecutive years.- Mentored and trained 3 Masters of Science students; passed on routine protein chemistry skills.

- Introduced students to techniques routinely used in a protein structure laboratory, passed on laboratory skill-set through hands-on experience.

Graded written assignments and exams, demonstrated experiments and carried out laboratory supervision for: - Introductory Inorganic Chemistry - Organic and Biological Chemistry - Biochemistry - Molecular Biology - Protein Discovery and Analysis

- Used recombinant protein protocols to express and isolate biomacromolecules localised within genomic islands in pathogenic Aceinetobacter baumannii. - Gained experience in initial matrix screens for protein crystallization.

- Designed, expressed and purified polyprotein fusions of RNA-binding proteins.- Carried out solution studies, using SEC and MALLS, to determine self-assembly and oligomerization pathways

Worked with the Respiratory & Environmental Epidemiology Team .Ambient air samples and respiratory air samples from human subjects were collected at locations surrounding the Lane Cove Tunnel, Sydney at various times of the day. These samples were analysed for N-containing gas compounds to determine if there is a relationship between pollutants from the tunnel and local respiratory health.