Francesca Papi Email & Phone Number

Siena, Toscana, Italia

As part of Ph.D I am acquire/improve the following skills:- Set-up of multiplex assays (Luminex assay)- In vitro assays to identify antigene specific functional antibodies (Luminex assay)- PCR to clone sequences of interest- Manipulation of in vitro cells suspension culture (e.g., Expi293 Expression System, CHO Cell Culture)- SDS page (enhanced competence)- Western blot (enhanced competence)- Small scale Abs purification- Basic knowledge of liquid Handler usage (KingFisher)- Basic knowledge of liquid Handler usage with Hamilton Workstation for assays Automatization

Siena, Toscana, Italia

As part of this Internship I am acquiring the following skills:- Transformation of chemically competent cells (Stellar, BL21 etc)- Molecular cloning techinques- Plasmid purification with appropriate kits- PCR to clone sequences of interest- SDS page- Western blot-In vitro assays to identify functional antibodies (Luminex assay)-Cell biology techniques for transfecting mammialian cell lines with recombinant monoclonal antibodies-Use of Quiagen CLC Genomics Workbench software for sequence analysis

Lisbon Area, Portogallo

Project title: "Study of the cilia dynamic distal domain in response to environmental changes in Tetrahymena thermophila cells"Supervisor: Prof. H. Susana Marinho, Prof. Helena Soares, Dr. Bruno Carmona As a part of this project I acquired the following skills:- Cell culture - Immunofluorescence staining- Microscope manipulation and images acquisition (using the ImageJ program to analyze microscope images and process them)- Preparation and development of experimental procedures, with adaptations needed for the experiment’s objectives. - Capability of critical analysis of experimental outcomes.

Doctor Of Philosophy - Phd, Biology, General, Eccellente

Master'S Degree, Molecular Diagnostics And Biotechnology, 110/110 E Lode

Thesis title: "Ciliates of the genus Euplotes as models for studying adaptation, response to stress and cell manipulation". Supervisor.

Bachelor’S Degree, Biosciences &Biotechnology, 95/110

Thesis title: "Experimental setup for the replacement of Escherichia coli 16S and 23S rRNA genes with the correspondent apicoplast genes.