Fraser Leslie work email
- Valid
- Valid
- Valid
Fraser Leslie personal email
Scientist with MS & 6+ years industry experience formulating LNP, characterizing, and independentlyevaluating in-vitro and in-vivo mRNA lipid nanoparticles.Optimized LNPs for scale-up (CDMO/CMC tech transfer) or scale-down (HTS screening) at 2 companies.Advanced cross-functional proficiency in LNP process development, assay development, immunology, andmouse work.
-
ScientistAverna TherapeuticsBoston, Ma, Us
-
Associate ScientistOrna Therapeutics Jan 2023 - PresentWatertown, Massachusetts, Us● Performed scale up optimization (formulation, purification, and filtration) with PNI Blaze and TFF for leadlipids for tech transfer to CDMO● Developed a Flow Cytometry based potency assay for the lead DS and DP, which included the writing ofthe SOP and Protocols● Developed a J2 dsRNA detection assay using luminescence or electrochemiluminescence (MSD),including writing a method procedures and SOPs● Investigated the stability of LNPs made at scale with Blaze and TFF using in-vitro expression, cryoTEM, and HPLC● Produced circular RNA (oRNA) and mRNA LNPs for internal or external programs and taught cross-functional teams proper LNP handling and storage● Developed a high throughput automated process for screening RNA with empty LNPs using the TECAN liquid handler -
Senior Research AssociateOrna Therapeutics Jul 2021 - Mar 2023Watertown, Massachusetts, Us● Led investigatory in vivo studies for the optimization of dose schedules, steroid pretreatment and dose escalation to measure their impact on tolerability, cytokines, and ADA (anti-PEG IgMs)● Screened an immunotropic lipid library through in vitro and in vivo models using FACS and IVIS; Independently designed study, formulated, characterized, handled mice (necropsy), processed tissue, and ran flow cytometry● Developed a scalable purification method for LNPs using Repligen TFF, which included in-process sampling, hold time stability measurements, and freeze thaw experiments● Characterized formulations using Zetasizer, osmometer, HPLC, Endotoxin, cryoTEM, and Ribogreen assay● Delivery team Lab Ops leader; Maintained Inventory, SOPs, ELNs, instruments and organized lab move to new locations
-
Research Associate, Formulation And Drug DeliveryOmega Therapeutics Jan 2019 - Jul 2021Cambridge, Massachusetts, Us● Performed initial scale up optimization of LNPs with static and chaotic micromixers● Formulated, characterized and optimized novel lipids into LNPs using the NanoAssemblr or Spark● Purified and analyzed DNA/RNA from liver and cell samples● Quantified editing (CRISPR) through enzymatic essays, Western blots, qPCR and FACS● Characterized transfection efficiency and cytotoxicity in multiple cell lines including primary mouse hepatocytes● Maintained Inventory, SOPs and ELN -
Formulation And Drug Delivery Co-OpOmega Therapeutics Jun 2018 - Dec 2018Cambridge, Massachusetts, Us
-
Graduate Research AssistantNortheastern University Aug 2017 - Jun 2018Boston, Ma, Us• Maintained and monitored multiple cell line for projects within the lab including cell counting, transfer and seeding• Evaluated miRNA reprogramming of tumor associated macrophages from pro-tumor to anti-tumoral phenotypes • Formulation of TAM targeted HA-PEI nanoparticles for in vitro and in vivo assessment of delivery and miRNA transcription• Analysis of nanoparticle biodistribution in-vivo through IVIS and measurement of tumor growth• ID8-VEGF syngeneic orthotopic Endothelial Ovarian Cancer model development and characterization -
Laboratory Research AssistantBinghamton University Jun 2016 - May 2017Binghamton, Ny, Us• Formulate PLGA polymer nanoparticles using water in oil in water emulsion method• Tested and classified nanoparticles using zetasizer, Scanning electron microscope and BCA assay• Employed nanoparticles with encapsulated antibiotic to target the growth of biofilms with success • Collaborated with the Biofilms Laboratory at Binghamton to monitor the performance between the encapsulated antibiotic versus free antibiotic -
Undergraduate Teaching AssistantBinghamton University Sep 2015 - May 2017Binghamton, Ny, Us• Supervised and assist 24-26 students, checking the prelab assignments and taking attendance• Communicate knowledge of the lab and providing feedback on how to improve the lab
-
InternDecodify.Me May 2016 - Aug 2016• Researched SNPs and their effect on a variety of health issues, including diabetes, prostate cancer and heart disease• Analyzed DNA strands of 10+ clients and complied lists of SNPs, ranking them based on severity
Fraser Leslie Skills
Fraser Leslie Education Details
-
Northeastern UniversityBiotechnology -
Binghamton UniversityBiomedical/Medical Engineering
Frequently Asked Questions about Fraser Leslie
What company does Fraser Leslie work for?
Fraser Leslie works for Averna Therapeutics
What is Fraser Leslie's role at the current company?
Fraser Leslie's current role is Scientist.
What is Fraser Leslie's email address?
Fraser Leslie's email address is fl****@****ton.edu
What schools did Fraser Leslie attend?
Fraser Leslie attended Northeastern University, Binghamton University.
What skills is Fraser Leslie known for?
Fraser Leslie has skills like Microsoft Office, Microsoft Word, Powerpoint, Microsoft Excel, Process Improvement, Project Management, Event Planning, Management, Leadership, Research, Training, Matlab.
Free Chrome Extension
Find emails, phones & company data instantly
Download 750 million emails and 100 million phone numbers
Access emails and phone numbers of over 750 million business users. Instantly download verified profiles using 20+ filters, including location, job title, company, function, and industry.
Start your free trial