-Methods Development, Qualification, and Validation of Molecular Biology assays with a focus on supporting Cell & Gene Therapy pipeline.-Lead on ddPCR instrument validation and commissioning.-Lead scientist on the development of ddPCR assay for genome titers to support Gene Therapy. Responsible for initial assay design (single and multiplex targets), writing of test method and protocols, and assay qualification.-Lead scientist on development of QF-PCR methods for PCR endpoint of TCID50 infectious titer assay as well as rcAAV PCR end point.-Responsibilities include writing protocols including Qualification and Validation studies, analyzing data, presenting data to internal and external clients, authoring reports, and instrument validation and commissioning.

-Methods Development, Qualification, and Validation of Molecular Biology assays with a focus on supporting Cell & Gene Therapy pipeline.-Lead on ddPCR instrument validation and commissioning.-Lead scientist on the development of ddPCR assay for genome titers to support Gene Therapy. Responsible for initial assay design (single and multiplex targets), writing of test method and protocols, and assay qualification.-Lead scientist on development of QF-PCR methods for PCR endpoint of TCID50 infectious titer assay as well as rcAAV PCR end point.-Responsibilities include writing protocols including Qualification and Validation studies, analyzing data, presenting data to internal and external clients, authoring reports, and instrument validation and commissioning.

-Methods Development, Qualification, and Validation of Molecular Biology assays to support client's in a contract research model.-Responsible for initial assay design and development, writing test methods and protocols, data analysis, leading qualification and validation studies, and training junior scientist on assay performance.-Lead scientist on several projects ranging from QF-PCR and RT-PCR as well as agarose gel based assays and Southern blots.

- Interact with clients and sales force to create experimental designs specific to new projects.- Perform Molecular Biology laboratory experiments, tests, and procedures following cGMP guidelines. These include isolation and analysis of DNA, RNA, and proteins. PCR, qPCR, RT-PCR, RT-qPCR, and DNA sequencing.- Provide technical guidance and training to less experienced technicians and personnel.- Monitor key project events and time lines.- Write documents, including protocols, amendments, memos, reports, and Excel/Word tables for data evaluation. - Provide quality control review of documents, reports, and data.

Primary Mouse Embryonic Fibroblasts (MEF) cellsMurine Embryonic Stem Cells for commercial salePoint person to assist Tech Services with customer related inquires and issues

Genetic Engineering - transgenic mouse linesMurine Embryonic Stem (ES) cell tissue culture Mammalian tissue culture (transient and stable transfection)Secretary of Radiation Safety CommitteeCell and Molecular Technologies, Inc. was acquired by Invitrogen, Inc. December 2006Promotions from Laboratory Assistant to Associate Scientist to Sr. Associate Scientist during my tenure

Responsible for Trace Metals testing of Raw Materials and Finished Products via ICP-AES, ICP-MS, and GFAAS by following SOP and Clean room protocols.Prepared daily standards and calibrations, maintenance of instrumentations, and created detailed reports of analysis for C of A.Assisted in development and implementation of LIMS data acqusition system.