John Garbutt Email & Phone Number

Union City, Ca, Us

Emeryville, California, Us

Bench Scientist in process development for upstream production of plasmid DNA for oncology and viral vaccines, using the plasmid DNA in In Vitro Transcription Reactions (IVT) to make RNA at different scales and downstream purification of RNA. Skills utilized are growth of E.coli in shake flask, extraction and purification of plasmid DNA, restriction digest of plasmid DNA, IVT to make RNA and column purification of RNA and TFF

San Francisco, Ca, Us

Cell Line Development and Protein ExpressionCell culture, multiple cell lines CHO, Hybridoma,HEK293. Analytical techniques ELISA, SDS-PAGE, Western Blotting, various protein purification/fractionation techniques, including tangential-flow filtration, column chromatography, analytical HPLC assays.

Manufacturing of Isotopic-metal conjugated antibodies, QC testing of raw materials, in-process materials and finished goods for release. Subject Matter Expert in the development and testing of new procedures• Performed biological experiments (cell culturing, cell isolation, cell staining)• Maintenance and sustaining of the cell bank• Performed product testing using standard ICP-MS and novel Mass Cytometry technology on CyTOF instruments• Worked with Reagent Manufacturing QC to determine quality product and collect production data• Executed manufacturing validation test plans, assists with the preparation of technical reports• Ensure lab equipment is calibrated in a timely manner and maintain quality records in the Quality Assurance database

Production of bacteriophage against Cutibacterium acnes (clinical bacterium causing acne)• Guidance and assistance to make Master Cell Bank and Working Cell Bank• Experiments to define optimum concentrations of components in growth medium to achieve optimal growth of bacterial culture (Increased growth 4X)• Testing MOI (Multiplicity of Infection) for amount of bacteriophage to infect host bacterium with to produce maximum yield of bacteriophage (Increased two logs)• Provided a bench scale clarification and filtration system (Centrifuge, PP3 1.2um-0.65um, XLG 0.2um)• Selection of TFF system for filtration, concentration and buffer exchange (GE AKTA FLUX) • Write-up of protocol for CMO to use to scale process from ml at bench to Liters in bioreactor

Franklin Lakes, New Jersey, Us

Execution of verification activities throughout all phases of the FACS product development process• Assist in design of test methods and test strategy• Development and execution of formal protocols• Analysis and interpretation of results• Communication of results and their impact through written reports, and interactive discussions• Conducted experiments in flow cytometry to define best laboratory procedures and study designs

Rahway, New Jersey, Us

Growth of Hybridoma cell lines to produce antibodies for characterization and scale up• Expansion of screened hybridoma cell lines to produce antibodies for characterization, analytical testing, and cell banking• Growth of rat and mouse hybridoma cell lines in T-Flask, Spinner tube, shake flask, Integra bioreactor and small scale bioreactor to produce antibodies for epitope binning, sequencing and kinetic analysis• Designed experiments for production, work flow, quantization by octet384 and initial purification. Documentation of work & results in database and electronic notebook with presentation of analyzed data to various groups

South San Francisco, Ca, Us

Cell line development in CHO to express Andexanet by transfection and electroporation• Optimized NEON electroporation conditions for vectors• Successfully generated CHO-S culture that had stable integration of linearized plasmid DNA• Selection of 10X producing cell line pools for Andexanet• Designed and planned out bioreactor laboratory• Selection of bioreactor system and supporting equipment• Configuration, Set-Up, Operation and harvest of 10L stirred tank• Documentation of data into reports, Power Point Presentations

San Francisco, California, Us

Production of M13 bacteriophage from Lab-Scale bioreactors to supply single-stranded DNA to fold and construct multiple DNA nanostructures.• Developed a bench-scale process to produce M13 from 2L bioreactors• Developed a recovery and purification process for M13 bacteriophage• Extract and purify M13 DNA• Assist in the folding of M13 template DNA into “DNA Origami Nanostructures”• Assisted in molecular biology, protein expression and strain development• Developed a qPCR assay for quantification of M13 DNA

Gaithersburg, Maryland, Us

Process development and cGMP clinical manufacturing of Live Attenuated Influenza Vaccine (LAIV). Establish and prepare Working Cell Bank, Virus Working Banks. • Thaw of WCB adherent MDCK & Vero cells• Expansion of cells in T-flasks and roller bottles with determination of parameters and timing of culture to ensure optimum growth• Set-Up, inoculation, control with data logging of 50L Single Use Bioreactors SUB and harvest of virus• Preparation of Master Production Records (MPR), Technical Reports and presentations

South San Francisco, California, Us

Process development for pilot plant scale fermentation of E.coli and conversion into cytoplasmic extract for cell free protein production. Supporting research on cell-free extract protein production technology.• Operation of bioreactors at 1L-10L-200L for growth of E.coli• Homogenization of E.coli cells to form extract• Centrifugation to differentially separate extract into active component• Running of assays for growth and production of recombinant proteins

Burlingame, Ca, Us

Process development for bioreactor growth of filamentous fungus Neurospra Crassa for production of recombinant proteins and antibodies• Set up, operation and harvest of Sartorius stirred tank (3L) bioreactors• Processing of bioreactor time course samples for wcw and dcw measurement of accumulated cell mass• Off-line analysis of glucose consumed and metabolites generated for media development• Feeding of nutrients for fed-batch bioreactor process to drive expression of proteins• Analysis of expressed proteins by SDS-PAGE and western blot• Preparation of technical reports and presentation of results to staff

Us

Cell Culture Process Development - Growth of mammalian cells to produce adenovirus, growth factors and antibodies.• Design and implementation of experiments to compare and screen cell culture media that would support optimal growth and expression of recombinant proteins from several different cell lines.• Batch Bioreactor Process Development for the production of recombinant proteins. Determination of Process effects of pH ranges and shifts, dissolved oxygen setpoints, inoculation, seed train and recovery.• Developed Fed-Batch Bioreactor Process that improved yield of antibody 4X over batch process by tailoring a feed strategy that optimized use of substrate with minimum accumulation of byproducts • Scale-up of Fed-Batch process from 3L to 1000L and use of scale-down methodology to trouble shoot the fed-batch process to fit schedule and capacity of GMP facility

Us

Cell Culture Process Development - Growth of mammalian cells to produce adenovirus, growth factors and antibodies.• Served on multiple project teams that Coordinated with Research and Development to deliver timely produced materials in support of multiple projects• Wrote numerous technical reports for process development, process transfer and SOP establishment for GMP manufacturing• Data from studies was analyzed with results presented in departmental meetings and scientific conferences• Use of HPLC systems (ProteinA and Size Exclusion Chromatography) to purify and measure antibodies

Us

Cell Culture Process Development - Growth of mammalian cells to produce adenovirus for Gene Therapy for Myocardial Ischemia• Shake Flask studies to determine optimal Multiplicity of Infection (MOI)• Shake Flask studies to select optimal media for growth of host cell and infection medium to produce virus• Preparation of cell banks (RCB) of HEK293 and Perc.6• Perfusion Bioreactor process development to produce high viability, high density inoculums to seed adenovirus production bioreactors• Continuous Batch bioreactor process development for production of recombinant adenovirus. Selection of optimum pH, temperature and dissolved oxygen. MOI

Supervision and coordination of lab scale 10-80L fermentations for the expression of recombinant proteins for therapeutic pharmaceuticals or viral vaccines.• Arranged and implemented documents (GMP) to direct and initiate fermentations as requested by multiple Chiron Project Directors.• Coordinated the preparation of fermentation components and media• Directed (hands on) seven operators and assisted in the set-up, inoculation, operation and recovery for fermentations of E.coli and yeast• Analysis of fermentation metabolites and off-gases• Computer filing and processing of fermentation data to spreadsheets and graphs.

Fermentation-Process Development and evaluation of bacteria expressing biological active proteins from both natural isolates and recombinant genes.• Developed Fed-Batch fermentation process which increased the yield 2X of expressed insecticidal proteins• Reduced the cost and increased potency yield by 20% of current manufacturing fermentations by improving carbohydrate utilization• Developed alternative media for fermentation of several different current and future products to fit EPA specifications and market demands.• Scaled-Up from shake flask to laboratory scale (30L) to Pilot Plant (750L) both new strains and process methodologies.

Research on the influence of various plasmid DNA shuttle vectors on growth and gene expression in Insecticidal bacteria.• Mapping of insecticidal protein genes by DNA endonuclease restriction analysis, DNA hybridization and PCR• Establish cellular mating system to transfer plasmid encoded genes• Developed and implemented unique methods and techniques to cure plasmid DNA from bacteria and the detection of high molecular weight plasmid DNA.• Developed bacterial host strain for expressing recombinant insecticidal proteins

Master Of Science, Molecular Microbiology

B.Sci, Microbiology