Gary Chaffee Email & Phone Number

Milwaukee, Wisconsin, United States

Chemist II in Synthetic Biology manufacturing in Amidites. Commissioned a large scale manufacturing lab. Updated/upgraded laboratory equipment, SOP's, PPE, and chemical manufacturing processes.• Large and small scale manufacturing of phosphoramidites using reactors and glassware. Other equipment used include chemical transfer hose, sanitary connections, columns, rotovap, vacuum pump, air stirrer, diaphragm pump, grinder/mill, sieve, sparkler filter, vacuum oven, and nutsche• Commissioned a large scale manufacturing lab containing multiple large scale reactors, large volume columns, solvent distribution system, vacuum pumps, vacuum ovens, chillers, and TCU’s• Trained in various skills such as, equipment inspection, reactor operation, and general laboratory skills• Head chair of the Holiday Party planning committee for the site• Shift and team lead in charge of running and communicating operation statuses of chemical processes as well as communication with other departments • Trained and mentored many employees in chemical processing and adapting to their roles• Involved with 5S committee• PPI (Practical Process Improvement) certified process manager• Perform internal 5S safety audits and quality checks• Create, update, and revise PR’s and OP’s, perform investigative development work on processes

Sheboygan, Wisconsin Area

Sr. Production Scientist in Stains and Dyes Production. Began my role in Milwaukee-Emmber facility and moved to the Sheboygan site to commission the new home of Stains and dyes manufacturing, PRO5. My position gave me industry experience with large and small scale chemical manufacturing. I also was responsible updating/upgrading SOP's, chemical manufacturing processes, equipment, and training new employees. Was 40 hour Hazmat trained for emergency response.

Whitewater, Wisconsin

Academic Research:• Dr. Chris Veldkamp, Associate Professor (262)-472-5267, veldkamc@uww.eduProtein synthesis, purification, analysis, mapping, and drug docking characterization• Protein expression and purification. Techniques regularly used included bacterial cell culture, bacterial transformation, centrifugation, cell lysis by sonication, immobilized metal ion affinity chromatography/nickel affinity chromatography, protein refolding through dialysis or infinite dilution, enzymatic digestion (to cleave affinity tags from the protein of interest), ion exchange chromatography, reverse phase HPLC, SDS-PAGE• General laboratory skill, including preparation of common reagent like buffers and media, cleaning and sanitation of lab-ware, monitoring supply levels, accurate recording of results in a lab notebook and general lab upkeep• Maintain or create standard operating procedures/protocols for production of recombinant chemokines and other proteins• Trained and supervise new students, new workers, and French foreign exchange interns in the labPublication:• “The solution structure of the forkhead box-O DNA binding domain of Brugia malayi DAF-16a.” • “Solution structure of the human chemokine CCL19”• “Polysialation of CCR7 controls dendritic cell trafficking by releasing CCL21 from an auto-inhibited state” – submitted manuscriptPosters:• "Expression and Purification of a CCL19/21 Chimera Protein in BL21 pREP4 Strain of E. coli"• "Chemokine CCL19/mCherry Fusion for Fluorescent Gradient Visualization"

Master Of Science - Ms, Biotechnology

Bachelor'S Degree, Chemistry Major And Biology Minor

Activities and Societies: Phi Mu Alpha Sinfonia National Fraternity, IFC, Chemistry ClubPhi Mu Alpha Sinfonia National Fraternity •.