Gemma De Ramon Francàs, Ph.D. Email and Phone Number

Ph.D. Thesis in molecular mechanisms of vesicular trafficking during neural circuit formation. - Proficient molecular biology and histology techniques (WB, ISH, HE, IHC, FISH, iRNA technology), in vitro experiments, microscopy, and data analysis. - Development, optimization, and establishment of protocols and techniques. Writing of SOPs and laboratory protocols.- Writing and publication of papers and reviews. Presentation of posters and small talks in several Scientific Conferences (ISDN2016, SSN meeting 2016-2019, and ZnZ symposia).- Project management experience. Supervision of students, teaching, and coordination of practical projects. Transfer of knowledge to new hires. - Talented in organization and time-management. Work under deadlines and proficient documentation competence.

- Review of the results of ongoing research and clinical projects. - Functional and structural characterization of plasma proteins and their clinical application. Work under established protocols and aseptic conditions.- Design of new experiments and improvement of laboratory techniques.

Project title: Development of a new therapeutic approach based on gene therapy viral vectors for Multiple Sclerosis treatment - Design and cloning of a recombinant protein - Production, purification, and titration of viral vectors (Adenovirus and AAVs, BSL2). - Characterization of viral vectors (RT-PCR and Western Blotting). - Preliminary study of the activity (cell culture) and potential immunotherapeutic proprieties of recombinant protein. -Experience in gene therapy for Multiple Sclerosis

Project title: Study of the relation of Alzheimer Disease (AD) and Type Diabetes II (TDII)Collaboration project in Miguel Chillon’s Research Group, CBATEG. - Purification and analysis (Q-PCR and Western Blotting) of AD-related brain proteins in wild-type versus triple transgenic mica model (3xTgAD). - Statistical analysis and interpretation of the results.

Project title: New Genetic Engineering techniques and Gene TherapyCell Division Group. Institute for Research in Biomedicine - Understanding the first steps of preclinical studies. - Cell culture of suspension cells, transfection of Drosophila cells with asl-GFP-plasmid and protein expression analysis. - Microinjection of Drosophila melanogaster embryos with the white gene and phenotype comparison between transgenic and normal drosophila. - Design of transgenic mice and transfection of human cells under supervision.