Gemma Stokes Email & Phone Number

Chandler'S Ford, Gb

Southampton

-Management of a team of design specialists to support the development of assays-qPCR primer and probe design for genotyping, gene expression and pathogen identification, using sequence analysis, assay design software and DNA folding software-Communication with internal and external collaborators to define requirements for the assays and to set timelines -Producing customer reports on the feasibility of their requested custom assay-Performing design reviews of catalogue assay to ensure continued conformity to design criteria as part of the post market vigilance.-Identifying improvements to the assay design pipeline-Leading projects to introduce new technologies, develop new products and generate IP.-Curating sequence data and mine public databases for sequence content periodically-Presentations in R&D meetings and to customers-Producing bioinformatics files and other research-related documentation (including technical summaries) in line with an ISO13485 quality management system.-Providing expert technical support to customers is also required.

Nottingham

Providing molecular biology input and expertise to assist with preparing regulatory documents for CE marking of AMD molecular diagnostic kits under the IVD Medical Device Directive 99/79/EC.Designing validation studies and analysing data. Writing product manuals. Providing assistance in molecular biology bioinformatics.Assisting the Quality Assurance Officer in working towards implementing the ISO 13485 quality management system.Enhancing the company portfolio of PCR reagents and molecular diagnostic kits by writing a series of product manuals, package inserts and editing both AMD and Willowfort websites.

Loughborough

Duties include• Design and validate pathogen detection TaqMan qPCR and RT-qPCR assays on the FELASA panel of agents for animal health monitoring as part of the Full Spectrum testing service, including BLAST searches, PrimerExpress and DNA MegAlign software use. validation and trouble shooting using the CFX96 Touch. • Design and validate SNP genotyping assays for genotyping mutant and transgenic rodents.• DNA and TNA extraction from faecal pellets, tissue, swabs and filters. RNA isolation from tissue.• Training and supervising technical staff. Communicating with staff at all levels, including presenting to admin staff and animal technicians, collaboration with International colleagues. Representing colleagues in my department at the employee forum at site and UK level.• Providing technical expertise and advice• Writing and reviewing work instructions, working under ISO 9001:2015• To work flexibly and prioritise routine testing when necessary• Calculating testing cost. Sourcing reagents and equipment Some of my achievements whilst working for Envigo include:• Collaborated on an extensive international project to develop, validate and launch a panel of over 70 diagnostic tests targeting pathogens which affect the health status of laboratory rodents. This • Boosted progress on PCR development projects by training and mentoring other team members to achieve improved results• Implemented new high throughput technology (KingFisher and Fluidigm EP1) into the routine testing lab to meets the increased demands for animal health monitoring, cutting the testing times.• Successfully orchestrated the entry of marmoset samples into the UK from Japan, liaising with the customer, CITES, customs officials and the Envigo Regulatory Affairs Associate

Nottingham, United Kingdom

• Production and testing of biopharmaceuticals using HPLC, western blotting, light microscopy, flow cytometry and platelet aggregometry• Analysis of data for use in reports and the company brochure• Writing Standard Operating Procedures

Leicester, United Kingdom

Duties included• To conduct genetic and biochemistry research on iron status in mice using fluorescent microsatellite genotyping, SNP genotyping, RT-qPCR, western blotting, iron assays, transferrin binding assays and sanger sequencing preparation• Data analysisAchievements included• Identified four regions of the mouse genome in an F2 cross, using genetic analysis, which potentially harboured genes affecting iron homeostasis• Characterised potential candidate genes by gene expression profiling using cDNA microarrays and RT-qPCR, together with SNP discovery by re-sequencing• Co-wrote a first author paper on the research and presented findings in a seminar to non-geneticists and a poster for the MRC Quinquennial Review

Msc, Molceular Genetics

Bachelor'S Degree, Biology And Chemistry