group Stefan Kubicek - PLACEBO lab - chemical screening

group Stefan Kubicek - PLACEBO lab - chemical screeningCRISPR/Cas9 screening, CHIPseq, ChIPmentation, chromatome analysis, cell culture, RNA & protein isolation, Western Blot, molecular cloning

standard cell culture of different cell lines, protein isolation, Western Blot, flow cytometry, immunofluorescence, preparing of reagents and consumables

Title of master thesis: „Biochemical and knockdown analysis of chorein, the protein responsible for chorea-acanthocytosis“standard cell culture of different suspension and adherent cell lines, cultivation of primary cells, protein isolation, Western Blot, flow cytometry, immunofluorescence, immunoprecipitation, RNA isolation, Q-PCR, restriction cloning, Gateway and InFusion cloning, transient transfection, preparation of irradiated mouse embryonic fibroblasts, handling of cell matrix, hematopoietic differentiation of iPS cells, cytospin, antibody titration, preparation and fractionating of post nuclear supernatant, transformation of competent E. coli

Internship „Expression of levanase in Lactobacillus plantarum“CD-Laboratory for Genetically Engineered Lactic Acid BacteriaUniversity of Natural Resources and Life Sciences, ViennaDepartment of Biotechnologyplasmid preps, restriction cloning, agarose gel electrophoresis, selection and screening for levanase positive clones, expression of recombinant protein in Lactobacillus plantarum

2.7.21013-5.7.2012, 1.7.2013-4.7.2013: tutor at practical course „Genetik und Molekularbiologie“19.3.2012-6.4.2012, 11.3.2013-27.3.2013, 7.10.2013-25.10.2013: tutor at practical course „Übung IIIA – Molekularbiologische Laborarbeiten“preparing of reagents and consumables, assistance in the development of appropriate study skills

Internship „Protein purification of RNA helicase mutants & functional analysis“University of Vienna, MFPL, Department of Biochemistry & Cell biology, Group Christina Waldsichprotein purification (dialysis, sonification, affinity chromatography), ATPase Activity Assay

Quality management and quality control25.7. – 2.9. 2005, 26.6. – 1.9. 2006, 23.7. – 24.8.2007, 9.7. – 3.8.2012manufacturing controls, incoming goods inspection, quality control of pulp and paper

Title of bachelor thesis: „Cloning of RNA helicase mutants“restriction cloning, PCR, transformation of competent E. coli, agarose gels, polyacrylamide gel electrophoresis, preparation of reagents and consumables