Gibran Holmes Email & Phone Number

Maryland

Strategic planning to drive growth of Western Blotting workflow reagents and Gel/Protein Blot Documentation Imagers within key accounts that include government, academia, and private/public industries. Utilizes distribution channel to supplement direct methods of marketing brand and product line.

Bethesda, Maryland

Strategical planned to increase laboratory sales within key accounts that include the federal government, academia, and private/public sectors that resulted in exceeding plan by 15% at the completion of my first calendar year (January to December) in 2015. Utilize manufacturing representatives to bring additional value to customers. Marketing/sales include, but not limited to, various plastic consumables, reagents, and equipment.

Maintain a 26-different strain mouse colony by genotyping mice and submitting requests for setting up breeders or euthanizing unneeded mice. Created an electronic and paper inventory to keep track of 26 strains of mice for two different section of the laboratory involving 11 investigators. Responsible of chemical and radioactive waste removal from laboratory and the maintaining of records. Trained summer students and post-bacculaureate research fellows on laboratory SOP's and Safety. Represent… Show more Maintain a 26-different strain mouse colony by genotyping mice and submitting requests for setting up breeders or euthanizing unneeded mice. Created an electronic and paper inventory to keep track of 26 strains of mice for two different section of the laboratory involving 11 investigators. Responsible of chemical and radioactive waste removal from laboratory and the maintaining of records. Trained summer students and post-bacculaureate research fellows on laboratory SOP's and Safety. Represent the lab as an NIAID Laboratory Safety Committee Member. Show less

Studied expression and phosphorylation of a focal adhesion protein paxillin in murine cells and its effects on cellular polarization and migration. Techniques involve are transfection of mammalian cells, cloning, immunoprecipation, Western blotting, FACS analysis and migration assays. Other laboratory techniques used for various projects are transforming bacteria, plasmid extraction, RNA isolation, culturing adherent and non-adherent established cell lines. Responsible for training laboratory… Show more Studied expression and phosphorylation of a focal adhesion protein paxillin in murine cells and its effects on cellular polarization and migration. Techniques involve are transfection of mammalian cells, cloning, immunoprecipation, Western blotting, FACS analysis and migration assays. Other laboratory techniques used for various projects are transforming bacteria, plasmid extraction, RNA isolation, culturing adherent and non-adherent established cell lines. Responsible for training laboratory personnel on procedures such as Western Blotting and mammalian cell culturing. Practice Quality Control for lab's mammalian cell culture, using an ELISA kit to test for mycoplasma. Negotiate with vendors on pricing and volume to adequately maintain lab supplies. Presented research at scientific conferences. Show less

Purified and concentrated proteins such as antibodies, antibody-substrate complexes, and membrane bound proteins for crystallization. Crystallized Fab portions of a cryoglobulin antibody using the vapor diffusion method. Presented research at scientific conferences.

Studied expression levels of cytochrome P450's in pesticide resistance Drosophila. Reverse transcribed mRNA, from Drosophila, to obtain cDNA of two specific cytochrome P450 genes. Amplified genes of interest and ligated them into vector pBOV. Bacteria cells were then transformed with pBOV carrier of genes of interest for additional studies.