Waggoner Center for Alcohol & Addiction Research

Waggoner Center for Alcohol & Addiction ResearchGenomics & Proteomics applied to the study of alcoholism. Alcohol-responsive protein networks underlying excessive ethanol consumption. Proteomic identification of novel targets for medication development in alcoholism. Role of microRNAs in alcohol research. Collaborated with different groups. Presented data in several international conferences. qRT-PCR, Western blotting, 2D-DIGE, immunoprecipitation, co-immunoprecipitation for interaction… Show more Waggoner Center for Alcohol & Addiction ResearchGenomics & Proteomics applied to the study of alcoholism. Alcohol-responsive protein networks underlying excessive ethanol consumption. Proteomic identification of novel targets for medication development in alcoholism. Role of microRNAs in alcohol research. Collaborated with different groups. Presented data in several international conferences. qRT-PCR, Western blotting, 2D-DIGE, immunoprecipitation, co-immunoprecipitation for interaction proteomics, principles of mass spectrometry, microarrays, analysis of data with bioinformatics. Show less

Successfully worked on different projects while managing and coordinating a small group of undergraduate students. Collaborated with different groups. Presented data in several conferences. Effects of ethanol on StAR protein expression in GABA(B)-R1 subunit knock-out mice brain. Effect of antidepressant drugs on serotoninergic and noradrenergic neurons sensibility on the response to stress or to anxiogenic drugs: Role of neurotrophic factors. GABA(A) receptor plasticity: the role of ethanol and… Show more Successfully worked on different projects while managing and coordinating a small group of undergraduate students. Collaborated with different groups. Presented data in several conferences. Effects of ethanol on StAR protein expression in GABA(B)-R1 subunit knock-out mice brain. Effect of antidepressant drugs on serotoninergic and noradrenergic neurons sensibility on the response to stress or to anxiogenic drugs: Role of neurotrophic factors. GABA(A) receptor plasticity: the role of ethanol and neuroactive steroids. Effects of vagus nerve stimulation on neurotrophins gene expression and cell proliferation in the rat brain. Rodent handling, husbandry, drug administration, behavioral testing, brain dissections, basic surgery. Biochemistry, Cellular and Molecular Biology: Rat cerebellar primary granule cells cultures, total RNA extraction, PCR, molecular cloning, isolation and purification of plasmids, radioactive 32P-labeled probes, RNase protection assays, Western blotting, immunohistochemistry, image analysis. Show less

Cellular and Molecular Technologies LaboratoryCellular Neurobiology LaboratoryToxicologyThese courses included theoretical and practical work. I organized and presented lectures regarding experimental techniques in molecular neurobiology, supervised laboratory activities and assisted students in their practical work. I was co-responsible for grading students in their final exams. These courses were attended by undergraduate and graduate students.

Molecular mechanisms of tolerance to and withdrawal of GABA(A) receptor modulators. Primary Cell Cultures and Molecular Biology techniques, Statistical analysis of data, handling of rodents.