Analysis of the behavior and gene expression of cells exposed to shear stress in an Ibidi® channel- Culture and stimulation of cells inside the Ibidi® channel- RNA isolation protocol - RNA sample measurment - microscopy analysis- RNA-sequencing analysis, RNA pathways analysis, data interpretation

Investigation of cell behavior stimulated with Hydrostatic Pressure in an high-throughput device- Create a user-friendly device, using Solidworks, to mechanically stimulate cells- 3D Print, solder, testing and troubleshooting valves, pressure sensors using Simulink- Cell culture, cell splitting, cell plating, Presto Blue analysis, fluorescent microscopy analysis- Statistical analysis, critical interpretation of the resultsMicrofluidic device fabrication to study the effect of biomechanical stimuli on HUVECs- Design and fabricate triple-membrane-layer microfluidic devices using AutoCAD and photolithography/ soft lithogrpahy techniques- Perform membrane displacement detection using a confocal microscope- Computational simulation of a PDMS membrane displacement and its intereaction with a fluidic environment using ANSYS- Reproduction of multi biomechanical stimuli that mimics the cardiovascular environment- Cell culture and cell stimulation on chip- Cell viability analysis

Course: BME 101 - Introduction to Biomedical Engineering- Guided and helped students during the entire course with Solidworks and Arduino- Graded assignments providing feedback on students' work- Answered to student's quesntion and doubts