Giulia Salvatore Email and Phone Number

Research activity in Dr. Giandomenico Russo's Molecular Oncology Lab, under the supervision of Dr. Maria Grazia Narducci. Understanding molecular mechanisms driving Sezary Syndrome (SS), an aggressive form of Cutaneous T-Cell Lymphoma (CTCL), by establishing long term culture of SS cells derived from patients' blood and organoid cultures derived from patients' skin.

I'm a dancer of the famous Italian Hip Hop Crew called Break Da Beat from October 2008, which won many contests in Italy and worldwide. From 2012 i'm also part of the Megacrew called Beat Kings, which placed for the second time as the Italian Champion.From 2011 i'm the choreographer of W.Beat Crew, an all-girls hip hop crew that exerts a great influence on the Italian hip hop scene.I'm also a teacher from 2011 for kids from 7 years old to adult.

Research activity at Interuniversity Consortium INBB, Science Department, Roma Tre University, Rome (Italy)LazioInnova Project: "Easy-To-use Immunization against Covid19 (ETICO)“ focused on the development of a nasal spray vaccine against Covid19 based on recombinant bacteria that elicit IgA production and CTLs activation in the mucosa.Collaboration with DOGMA LazioInnova project: "Design Of class-G Monoclonal Antibodies for the treatment of cutaneous T-cell lymphomas" aimed at the development, through computer-aided design, of mAbs (and CARs) able to recognize the TCR specifically expressed on the surface of the neoplastic cells.

Research activity in the laboratory of Prof. Simona Frontoni, Department of Systems Medicine, Faculty of Medicine, University of Rome Tor Vergata, Rome (Italy)Understanding the role of Müller cells in retinal neurodegeneration, and the influence of hyperglycemia and glycemic variation on Müller cells activation and potential reprogramming.

Research activity in the laboratory of Histology and Embriology of Prof, Massimo De Felici, Department of Biomedicine and Prevention, Faculty of Medicine, University of Rome Tor Vergata, Rome (Italy)Understanding the potential of Mesenchymal Stromal Cells-based therapies for female fertility preservationJob-related skills:- Cell culture maintenance and propagation; - Production of primary cell cultures from human and murine tissues;- Reprogramming of somatic cells into iPSCs;- iPSCs cell culture mantainance, propragation and differentiation;- Cell viability/proliferation/death assay; - Cell transfection;- Maintenance of murine colonies- Mice handling and restraint (including ability to perform intraperitoneal, intravenous, intramuscular, subcutaneous and intradermal injections and oral gavage);- Intraovarian transplantation of a cell suspension in laparoscopy;- Basic surgical skill of wound closure;- Harvest of organs from adult/newborn mice and murine embryos;- Harvest of 2-cell embryos from the oviduct of a superovulated mice;- Organs and tissues inclusion in OCT and sectioning with cryostat; - Histochemical, Immunohistochemical and Immunofluorescence methods on cells/tissues; - DNA, RNA and protein extractions from cells and tissues; - Real Time PCR;- RT-PCR;- Agarose and polyacrylamide gel Electrophoresis; - Western Blot;- Good ability to write projects and to write and present reports.

PhD course in Molecular Medicine and Applied Biotechnologies, in the laboratory of Histology and Embriology of Prof, Massimo De Felici, Department of Biomedicine and Prevention, Faculty of Medicine, University of Rome Tor Vergata, Rome (Italy)PhD Projects: - Development of a protocol to obtain human Primordial Germ Cells-like Cells (hPGCLCs) from human Mesenchymal Stromal Cells (MSCs)- Assesment of the capacity of MSC in situ or systemic transplantation to restore fertility in a mouse model of chemotherapy-induced Premature Ovarian Failure (POF)

Techniques:cell culture, proliferation essays, viability essays, SDS-PAGE, Western-blot, ELISA, FACS, protein extraction, mice immunization, mAb purification, production of mAb.