87) Livestock and microbiological risk (a review). Allevamenti e rischio microbiologico. Giuseppe Zicari, Vincenzo Soardo, Daniela Rivetti, Elena Cerrato, Domenico Russo. Ig. Sanità Pubbl. n. 5, 2013.88) La gestione delle deiezioni zootecniche: il rischio chimico (review). The management of livestock manure: chemical risk (review). Zicari Giuseppe, Soardo Vincenzo, Rivetti Daniela. Progress in nutrition n. 2/2013.89) Zicari Giuseppe, Soardo Vincenzo, Berruti Renza, Panata Marisa, Cerrato Elena. La modulazione della Tariffa per l’accesso al servizio di distribuzione delle acque potabili quale strategia per salvaguardare la risorsa idrica, in Provincia di Asti (Tariff modulation for the access to the drinking water system as strategies to the water resource safeguard in the Province of Asti (Italy)). Progress in Nutrition 2014; 16.

79) La coltivazione di Agaricus bisporus e alcuni aspetti ambientali e sanitari (The cultivation of the mushroom Agaricus bisporus (Champignon) and some environmental and health aspects). Giuseppe Zicari, Daniela Rivetti, Vincenzo Soardo, Elena Cerrato. Ig. Sanità Pubblica n.3 maggio/giugno 2012; 68: 435-446.80) La digestione anaerobica ed i rischi ambientali (Anaerobic digestion and environmental risks). Giuseppe Zicari. Terra e Vita ilsole24ore, n. 39 di settembre 2012.82) Funghi eduli e rischio chimico (Edible mushrooms and chimical risk (Review)). G. Zicari, D. Rivetti, V. Soardo, E. Cerrato, M. Panata. Progress in Nutrition vol. 14, N. 2, 2012.83) La coltivazione dei funghi Agaricus bisporus (Champignon), (The cultivation of mushroom Agaricus bisporus (Champignon), (Review)). G. Zicari, D. Rivetti, V. Soardo, E. Cerrato, M. Panata. Progress in Nutrition vol. 14, N. 3, 2012.84) Creosote released from railway-ties recycled and the sanitary risks. Giuseppe Zicari, Giuseppe Allegro, Domenico Russo, Daniela Rivetti, Vincenzo Soardo, Elena Cerrato. Ig. Sanità Pubbl. n. 2, 2013; 69: 203-222.85) The weeding of Ambrosia artemisiifolia and sanitary risks (Il diserbo di Ambrosia artemisiifolia e rischi sanitari). Giuseppe Zicari, Vincenzo Soardo, Daniela Rivetti, Elena Cerrato, Domenico Russo. Ig. Sanità Pubbl. n. 3, 2013; 69: 341-347.86) Livestock farming and atmospheric emissions. Giuseppe Zicari, Vincenzo Soardo, Daniela Rivetti, Elena Cerrato, Domenico Russo. Ig. Sanità Pubbl. n. 4, 2013; 69: 445-457.

Job description of consultations conducted in more than 10 years of work:- Applied the HACCP (hazard analysis and critical control points) for management of food security in different food manufacturing (6 years).- He has worked with several companies and organizations, the compilation of projects for funding (forscientific research projects) that have been approved (two years).- Implemented consulting for quality management and ISO (9000: process quality; 14001: Environment (Environmental Auditor qualified CEPAS); 17025: analytical tests), in companies in the sector of food and manufacturing (4 years).- Carried out consultancy in the field of accident prevention, fire prevention and management of environmental issues (water, waste, emissions) in different types of business (food and manufacturing), (5 years).

It has been a consultant to the Health Service of the Piedmont Region dealing with the assessment of risks arising from the management of waste, from the production of renewable energy, from livestock and industrial activities aimed at producing food packaging or food. Co-authored of the health risk assessments, training of health workers and participated in the management of procedures for obtaining provincial administrative permits on the part of private entrepreneurs (3 years). He has worked on regional projects (Health Service of the Piedmont Region and the Region of Emilia Romagna) for improving the management of environmental issues with implications for the health, led to the drafting of regional guidelines on the following topics (4 years): - Management of municipal solid waste, composting and health risks; - The anaerobic fermentation of manure and agricultural plant biomass for energy production (biogas plants) and health risks; - Pig farms and poultry and health risks; - The mining operations (quarries) and health risks.

He has participated or has been a speaker at over 100 conferences, seminars, courses and national and international conferences among which we highlight the XV International Congress organized by the National Order of Biologists and held in Umag (Croatia), on days 3 to 5 October 2002 entitled "Scientific progress, ethics, protection of resources: professional challenges of the third millennium "(details in annex of 11 pages that you send on request).Detail downloaded from the website: https://sites.google.com/site/zicari73/home.In 2012, for 5 weeks was in Florida and has participated in an International Conference on Food Security. During this time he attended the University of Florida and an intensive course of English language.

Training graduates and workers: Companies, Associations, Schools Master University.He has performed and / or organized training courses on various topics. See the websitehttps://sites.google.com/site/zicari73/home for more details.

He has worked with several publishing houses taking care of the preparation of informative articles on various topics including the following: food hygiene, pesticides, disinfection, certification, and quality of products, processes and services, epidemiology of foodborne diseases, safety at work, analytical problems.He has published more than eighty works of technical and scientific articles, three books, software and databases.More details and some publications can be downloaded free of charge (eg books) from: https://sites.google.com/site/zicari73/home

He collaborated on the creation and implementation of on-line information systems registration and cataloging of foodborne illness and the management of environmental issues for the Piedmont Region (2 years). He has worked on research projects with the University of Eastern Piedmont, with the University of Turin and the Piedmont Regional Health Service on the supervision of Food Transmitted Diseases (2 years).

Chemical analysis of pesticides, quality control, microbiological analysis of food matrices and water. Techniques applied: Quality Control - Determination of oleic acid olives; Degrees Brix and acidity of citrus and grapes; Determination of anthocyanins oranges Using Datatrace Temp System interface able to programmed via software small sensors that measure the temperature, to optimize the heat treatment during the cycle of production of preserves canned food; determination of chemical oxygen demand, oxygen of dissolved chlorine on sewage; physical and chemical analysis of the oil and milk. Determination of moisture, ash and gluten in the flour. - Analysis Pesticides - Methods for the extraction of these residues of pesticides than fruit and vegetables, from olive oil and water: Phosphorates, Pyrethroids, Carbamates, Acaricides, Benzimidazole, Dithiocarbamates, Triforine, Regulators and Growth; Using the gel filtration-permeation of citrus fruits and vegetables. Methods for the determination of the aforementioned molecules using techniques Gas chromatographic analysis with detector electron capture and nitrogen and phosphorus; Enzyme immunoassay for the detection of 2,4- Dichlorophenoxyacetic by immunoperoxidase; HPLC (High Pressure Liquid Chromatography) with detector UV-Visible or with derivatisation and fluorimeter for Carbamates. Microbiology - Determination of total coliforms, Salmonella, and total mesophilic bacteria on land selective solids in milk and dairy products. Microbiological analysis of water. Microbiological analyses of wastewater from livestock and Organic Fraction of Municipal Solid Waste.

He has worked in molecular biology laboratories in several public research institutes (3 years).TECHNIQUES: artificial insemination, microinjection, ginogenesi, electroporation, shootingtime-laps on the teleost Brachydanio rerio; Use cryostat; Immunolocalization by immunoperoxidaseand immunofluorescence staining hematoxylin and eosin; Amplification of DNA (deoxyribonucleic acid)by PCR (polymerase chain reaction); Insertion of DNA fragments into suitable vectors (enzimatca digestion, dephosphorylation, ligation), preparing competent cells of Escherichia coli, transformation; Plasmid Extraction and purification with the following methods: in gradient ultracentrifugationof cesium chloride, Qiagen method and the boiling method (to obtain small quantities); Electrophoresis of DNA digested with endonuclease; Quantitative analysis of DNA spectrophotometer; transfections in vivo tissue muscle (cardiac, soleus and EDL mouse), analysis of the expression of the reporter gene (Chloramphenicol test Acetyl transferase, luciferase and b-galactosidase).FISH (fluorescence in situ hybridization), cytogenetic prenatal c/o St. Orsola Hospital in Bologna.Test mutagenesis and carcinogenesis in vitro, Institute for Cancer Research of Bologna. Finger printing of DNA (PCR and gel electrophoresis policrilamide) extracted from the blood drop, Institute of Forensic Medicine, Pavia.

Ha lavorato in laboratori biologia molecolare in diversi Enti di ricerca pubblici (3 anni).TECNICHE APPLICATE: Fecondazione artificiale, microiniezione, ginogenesi, elettroporazione, riprese time-laps sul teleosteo Brachydanio rerio; Uso criostato; Immunolocalizzazione mediante immunoperossidasi e immunofluorescenza, colorazione ematossilina eosina; Amplificazione di DNA (acido deossiribonucleico) mediante PCR (reazione a catena della polimerasi); Inserzione di frammenti di DNA in opportuni vettori (digestione enzimatca, defosforilazione, ligazione), preparazione cellule competenti di Escherichia coli, trasformazione; Estrazione plasmidi e purificazione con i seguenti metodi: ultracentrifugazione in gradiente di cesio cloruro, metodo Qiagen e metodo della bollitura (per ottenere piccole quantità); Elettroforesi di DNA digerito con endonucleasi; Analisi quantitativa di DNA allo spettrofotometro; Transfezioni in vivo su tessuto muscolare (cardiaco, soleo e EDL di topo), analisi dell’espressione dei geni reporter (test Cloramfenicolo Acetil Trasferasi, Luciferasi e -Galattosidasi).FISH (ibridazione in sito con fluorescenza), citogenetica prenatale c/o Ospedale S.Orsola di Bologna; Test di mutagenesi e cancerogenesi in vitro, Istituto di Cancerologia di Bologna; Finger printing di DNA (PCR ed elettroforesi su gel di policrilamide) estratto da goccia di sangue, Istituto di medicina legale, Pavia.