Glen Monroe Email & Phone Number

Amsterdam, North Holland, Netherlands

Field Application Scientist

Leiden, South Holland, Netherlands

• Leading next-generation sequencing activities for product characterization• Leading implementation of new technologies (i.e. digital PCR) for method development and validation• Led investigations into analytical method transfer activities for the Janssen COVID-19 vaccine to CMOs in the Netherlands (Janssen Biologics B.V.); liason of molecular bioassay deliverables to external international sites (Germany, USA) • Subject matter expert (SME) for several molecular bioassays and provided global support for method updates and investigations• Authored SOPs, validation plans/reports, investigations, memos, etc.• Managed molecular bioassay deliverables regarding new vaccines for clinical use

Utrecht Area, Netherlands

• WGS / RNASeq of granulosa cell tumors and matched germline DNA to identify recurrence drivers and define treatment.• Establishment and propagation of patient-derived organoids and 2D cell lines; automated drugscreens to evaluate current chemotherapy/hormonal treatment and novel compounds. • Development of a CRISPR/Cas9 targeted nanopore sequencing to detect fusion genes.

Utrecht Area, Netherlands

“Discovering the genetic cause of Mendelian disorders in the age of genomics: the evolvingcapability of next-generation DNA sequencing” (Thesis date: 24 November 2017)By analyzing the genetics of affected individuals via target panel sequencing, whole exomesequencing, or whole genome sequencing, I interpreted variants and established causalitywith cell systems, relevant molecular biology experiements and model organisms (zebrafish).

Utrecht Area, Netherlands

Technical laboratory work in next-generation sequencing on the SOLiD and Ion Torrent platforms as well as for Illumina SNP genotyping and CNV analysis. Analysis of mendelian rare 'orphan' diseases as well as for large cohorts of patients with diseases of complex inheritance patterns.

New Haven Connecticut

Quality control for research and production DNA sequencingClinical genotyping and sequencing genetic test production analysis Haplotype construction for research and development

DNA extraction and isolation from a variety of sources DNA normalization, dilution, and array manually and by robotic platformsQuantity and quality analysis of isolated products using gel electrophoresis, UV spectrophotometer, and spectrofluorometerFamiliarity with PCR, DNA sequencing and MALDI-TOF genotyping. Familiarity with CLIA and HIPAA regulations and laboratory SOPs Maintained internal sample banking system

South Pole, Antarctica

Construction of the South Pole Telescope and new United States South Pole Station

Reviewed Material and Safety Data Sheets (MSDSs) for Loctite Products

Research SNP and indel discovery and population variation characterizationHaplotype construction for association in clinical studies, drug development, agricultural breeding and genetic variation researchPrepartion of gene research papers for external clientsPoint individual and management for QC projects; employee trainingPatents:April 2002 Haplotypes of the SCYB6 GeneMay 2002 Haplotypes of the ADORA3 GeneAugust 2002 Haplotypes of the CYP27B1 Gene

Doctor Of Philosophy - Phd, Human/Medical Genetics

Master Of Science, Biotechnology, Medical Specialization

Bachelor Of Science (B.Sc.), Cell/Cellular And Molecular Biology