Matt Barnette Email & Phone Number

Austin, Texas, Us

Austin, Texas, Us

Austin, Texas, Us

Austin, Texas, Us

Austin, Tx, Us

Accurately executes complex laboratory protocols for multiple next generation sequencing platforms including creating next-generation sequencing libraries (DNA, RNA, and metagenomic), operating/troubleshooting the Illumina MiSeq and NextSeq next-generation sequencers, operating the cBOT for HiSeq cluster generation, and some knowledge of how to prepare reagents, wash, and setup HiSeq 2500 next generation sequencers. Key Contributions:• Helped to develop and write complex protocols for High-Throughput DNA, RNA, and metagenomic library preparations, and qPCR that increased the number of samples that can be prepared at once while reducing the total turn around time. • Using Microsoft Excel and Visual Basic, wrote templates and macros for qPCR, library prep, and Covaris shearing to accompany high-throughput protocols to help reduce the amount of time spent manually organizing runs and performing in-protocol and result calculations.• Operate and help to maintain a large variety of laboratory equipment including Rainin Liquidator 96 well pipettes, Blue-Pippin, Covaris, QuantStudio qPCR machine, ViiA-7 qPCR machines Hamilton robot, Beckman FX robot, Beckman NX robot and Agilent Bio-Analyzer.• Certified by Tecan to program and operate the Freedom EVO and EVOware standard edition software after attending training in North Carolina in September 2016.• Able to quickly learn, execute, and troubleshoot new protocols with little to no errors.

Austin, Tx, Us

Austin, Tx, Us

Used acquired skills to perform DNA sequencing, fragment (STR) analysis, and train new students/employees in the use of various robots and real-time machines in a high output, customer service oriented environment.Key Contributions:• Maintaining and troubleshooting the ABI 3730 and 3730xl automated sequencers, ABI 7900 Real-Time PCR machines, ABI 7500 Real-Time machine, ViiA7 Real-Time PCR machines, Life Technologies Quant studio Real-Time machine, Biomek NX and FX robots, and the Qiagen Qiagility robot.• Consulting customers to help troubleshoot sequencing problems (such as secondary structure and concentration issues) in order to produce the best results possible, and maintain a good working relationship.• Assist in developing and writing experimental protocols such as the creation/use of a PCR cleanup sequencing control as well as “how to” procedures used as training aids for real time PCR machines. • Train new employees and grad students in DNA sequencing, Real-Time PCR, the use of the Biomek FX, Biomek NX, and the Qiagen Qiagility robots.• Lead tours of our DNA sequencing, robot, and real time PCR facilities to everyone from prospective faculty members and grad students, to undergrads and visitors.

Quickly learned and implemented many molecular biology techniques (PCR, real-time PCR, genotyping, automated DNA Isolation, automated DNA sequencing) in the customer service oriented environment of a core facility lab.Key Contributions: • Quickly learning how to use and troubleshoot the ABI 3130xl automated sequencer and the ABI BigDye Terminator v3.1 chemistry using manuals and resources to fulfill an immediate need in the core.• Troubleshooting genotyping assays to improve the quality of results and decrease turn around time• Train new employees in the techniques of tissue digestion, automated DNA isolation, genotyping, real-time PCR based genotyping using both the ABI 7300 and ABI 7900, and automated sequencing • Responsible for the ordering and inventory of the Bio-Rad Freezer program that serves the entire campus.• Nominated for and received an MD Anderson performance award for DNA sequencing work.

B.S. In Biochemistry, Biochemistry Option Ii: Computation

Certificate, Elements Of Computer Science