Ganapathy Sarma Email & Phone Number

Alameda, California, Us

Redwood City, California, Us

Cambridge, Massachusetts, Us

Cambridge, Massachusetts, Us

South San Francisco, California, Us

• Optimized conditions for site-specific, chemical conjugation of linker-payloads to monoclonal antibodies through collaborations within the company.• Initiated, optimized and developed methods for GLP- and GMP-scale conjugation and purification of different classes of linker-payloads to antibodies. • Developed, wrote and reviewed batch records, tech transfer documents, master procedures and development reports.• Worked closely with medicinal chemistry group on linker-payload design.• Closely involved in the transfer of optimized conjugation methods to manufacturing.• Successfully delivered high-quality results within time and budget for exploratory projects.• Mentored and trained research associates and scientists on conjugation and purification projects.

Lawrence Township, Nj, Us

• Initiated and developed methods for site-specific conjugation of drugs to monoclonal antibodies through collaborations within and outside the company.• Implemented structure-based modeling and docking methods for rational design of small molecules and novel sites for site-specific conjugation.• Designed mutant antibodies and enzymes for enzyme-based conjugation using homology modeling and protein-protein docking. • Successfully delivered high-quality results within time and budget for exploratory projects.• Recognized for outstanding performance in research and invited to present at BMS ADC meetings.

La Jolla, Ca, Us

Advisor: Dr. Susan S. Taylor• Initiated structural, biophysical and biochemical studies of D-AKAP2, an anchoring protein for cAMP-dependent protein kinase (PKA).• Determined the crystal structure of PKA regulatory subunit I in complex with D-AKAP2 using the weak anomalous signal from inherently present sulfur atoms by single wavelength anomalous diffraction (SAD).• Designed various D-AKAP2 constructs for structural and biophysical studies using bioinformatics, structure prediction programs, H/D exchange mass spectrometry and limited proteolysis.• Implemented biophysical and biochemical assays such as surface plasmon resonance, fluorescence polarization, and H/D exchange mass spectrometry to test and measure the interactions of shorter D-AKAP2 constructs with interacting proteins (Rab GTPases, PDZ proteins and PKA).• Discovered and defined the molecular code of AKAP specificity for the regulatory subunits of PKA, which is now the basis for designing peptide inhibitors in heart disease.• Discovered the mechanism of D-AKAP2 anchoring of PKA to the membrane by determining the ternary complex structure of D-AKAP2 with PKA and PDZ proteins. • Trained and mentored postdoctoral fellows, graduate and undergraduate students on research projects.

Corvallis, Or, Us

Advisor: Dr. P. Andrew Karplus• Determined the structures of two antioxidant proteins – glutathione reductase (GR) and antioxidant protein (AOP) from the malarial parasite, Plasmodium falciparum.• Demonstrated that a cavity in PfGR could be a potential binding site for parasite-specific, non-competitive inhibitors and helped characterize the effect of various small molecules on its activity. This work provided the foundation for the development of a clinical, phase II trial anti-malarial drug.• Elucidated the mechanistic details of peroxide binding to PfAOP and its inactivation by over-oxidation.• Determined the crystal structure of fungal toxin, Pyrenophora tritici-repentis, Ptr ToxA using sulfur SAD phasing and its oligomerization state using dynamic light scattering, sedimentation equilibrium ultracentrifugation, analytical gel filtration (thesis research).• Proposed a mechanism for toxin recognition by an integrin-like membrane receptor, internalization and transport of the toxin to chlorophyll based on careful structural analyses. • Carried out detailed data analyses to study the effects of resolution, data redundancy and crystal decay on the ability to use sulfur atoms for SAD phasing.• Provided training to graduate and undergraduate students on various crystallography techniques.

Doctor Of Philosophy (Ph.D.), Biochemistry And Biophysics

Master’S Degree, Biochemistry

Bsc, Biochemistry And Botany