Grace Freke Email & Phone Number

London, England, United Kingdom

• Performing molecular and cell biology work in the Genetics and Genomic Medicine department at the UCL Great Ormond Street Institute of Child Health, including:
• Design, optimisation, and execution of wet-lab experiments, to evaluate key hypotheses
• Techniques employed: CRISPR/Cas9 gene editing in mammalian cell lines, PCR, RT-qPCR, Western blotting, immunofluorescence and RNAseq
• Statistical analysis of complex experimental datasets (e.g. RNAseq), and visualisation of results, using Microsoft Excel, GraphPad Prism and R
• Presentation of work at lab meetings, departmental meetings, and progress meetings with international collaborators
• Drafting and circulation of paper manuscripts and progress reports

Stevenage, United Kingdom

• Industrial placement completed in the Cell Biology branch of the R&D Target and Pathway Validation group, during my PhD training.
• Used a range of medium- and high-throughput technologies, including Peggy Sue capillary-based Western blotting, IncuCyte in-incubator live-cell imaging and INCell Analyzer 6000 high-throughput confocal imaging
• Thoroughly documented experimental aims, methods, reagents, datasets and results in electronic lab notebook entries
• Attended group meetings to gain insight into the early-stage pharmaceutical R&D process
• Contributed suggestions and insight to projects outside of my own
• Presented my research within various levels of the company, including to the President of R&D

London, England, United Kingdom

Part-time work alongside postgraduate study.Oversaw day-to-day running of the three bars within the venue and ensured Premises License is upheld. Duties included managing bar, kitchen, security and stewarding teams, liaising with clients to ensure success of functions, stock counting and till reconciliation.Obtained a Personal License to authorise sale of.

London, England, United Kingdom

Part-time work alongside postgraduate study.Carried out Bar Staff duties, changed kegs and casks, cleaned beer lines, organised breaks of bar staff, oversaw restocking of bars, performed stock checks.

London, England, United Kingdom

Part-time employment alongside undergraduate studies.Set bar up before service began, took orders and payment, served food, prepared and served drinks, restocked bar, cleaned dining area and bar after closing.

Kent, England, United Kingdom

Part-time job while I studied for my AS/A-Levels.Taught Key Stage 2 English and mathematics, Key Stage 3 mathematics and pre-GCSE French. Managed time well to achieve learning objectives within tutorials, being creative in order to engage young pupils.

Otford, England, United Kingdom

Part-time job while studying for my AS/A-Levels.Took payments, ordered and managed stock, received and dispensed prescriptions in presence of pharmacist, liaised with local Medical Practice, gave product advice. Ensured patient confidentiality.

Doctor Of Philosophy - Phd, Molecular Cell Biology

Thesis title: Probing the Role(s) of Bbs1 with CRISPR/Cas9 Gene Editing Funded by a 4-year BBSRC CASE studentship sponsored by GSK.

Master Of Research (Mres), Systems And Synthetic Biology, Distinction

Thesis title: Measuring the Effect of Direct Mechanical Stimulation on GPCR Activation • Completed a 9-month laboratory-based research.

Bachelor Of Science (Bsc), Biochemistry, 2:1

Final year project: Developing a computational method to measure haematopoietic stem cell-to-osteoblast distances in wildtype and MPL.