Graham Holt Email & Phone Number

Chapel Hill, Nc, Us

Raleigh-Durham-Chapel Hill Area

• Developing distribution-based representations of protein structure and applying these representations to ML protein structure prediction and structure generation.

Raleigh-Durham, North Carolina Area

• Developed algorithms for computational protein structure design with the Donald Lab as a PhD Candidate in the Computational Biology & Bioinformatics program.• Applied computational tools to optimize protein-protein interfaces (PPIs) with a focus toward antibody-antigen interactions.

Washington D.C. Metro Area

• Developed molecular tools to measure and control neuronal activity with the GENIE team.• Designed high-throughput in vitro screening protocols for fluorescent genetically encoded calcium indicators (GECIs).• Cloned, purified, performed protein biochemistry assays, and analyzed data for ~1000 calcium indicator variants.• Worked with the Schreiter Lab to explore methods for activity and light-dependent genetic control of neurons.

Hanover, Nh

• Performed exploratory research on formins, proteins critical for regulation of the cellular actin cytoskeleton, in the Higgs Lab in the Biochemistry Department.• Characterized formin FMNL3 via alanine scanning and fluorescence microscopy. Completed honors thesis and presented poster, "Examining Mechanisms of Filopodia Assembly Via Mutation of FMNL3,” at Dartmouth’s Wetterhahn Science Symposium. Funded by Junior Research Scholarship.• Constructed phylogenetic tree of formin proteins and noted several highly conserved domains in formin INF2. Presented poster, "Sequence Alignment and Phylogeny of Domains in Formin Proteins,” at Dartmouth’s Karen E. Wetterhahn Science Symposium. Funded by Sophomore Science Scholarship.

Berkeley, Ca

• Evaluated transcriptional response of XPG to double-stranded break DNA damage using qRT-PCR and Western blotting in the Cooper Lab with Dr. Kelly S. Trego.• Completed research paper and presented poster entitled "Transcriptional Regulation of XPG in the DNA Damage Response."

Doctor Of Philosophy - Phd, Biomathematics, Bioinformatics, And Computational Biology

Bachelor Of Arts (Ba), Cum Laude, With Honors, Biomedical Engineering

Activities and Societies: Elected to Tau Beta Pi Honor Society, Dartmouth Ski Patrol