Grayson K. Email & Phone Number

San Francisco Bay Area

South San Francisco

• Generated sequencing data from monoclonal Hybridoma populations to support downstream R&D departments.
• Processed samples using RNA purification and RT-PCR amplification kits to prepare for submission to a vendor for sequencing services.
• Analyzed and compiled vendor generated sequencing data to guide project teams for hit selection with subsequent follow up to assist antibody engineering and drug conjugation workflows.
• Optimized pre-existing Beckman Coulter Biomek FX tissue culture methods and provided training for other employees.
• Developed and validated a progression of methods to complete an automated ELISA on a TECAN EVO200, Liconic Carousel and Hydrospeed washer.
• Developed and validated single step and two step RT-PCR methods using the Hamilton STAR and Formulatrix Mantis with LC3.

South San Francisco

South San Francisco

• Provides guidance and support for post-doctoral fellows and graduate students.
• Leads the team in the development and implementation of qPCR assays to determine information on viral replication patterns.
• Utilizes HEK-293T transfection systems for generating replication competent and/or pseudotyped lentivirus.
• Generates stable expression cell lines via pseudotyped letiviral integration systems.
• Implements cell viability and drug toxicity assays.
• Collects data from live and fixed cells on flow cytometers, and performs analysis of exported files with FlowJo.

South San Francisco

• Extremely proficient in isolating high quality plasmid/genomic DNA and total RNA from E.Coli and mammalian cells using Machery Nagel and Qiagen kits.
• Utilizes RioRad, Qiagen, Ambion, and Applied Biosystem SYBR Green and TaqMan Quantitative Real-Time PCR technologies to generate gene expression libraries and to perform gene copy number analysis with the Applied.
• Provides high throughput cell handling support for partner collaboration projects by subculturing primary mammalian cells and cell lines for usage in vitro screening efforts, and monitors growth patterns using the.
• Actively participates with the design, optimization, implementation, and analysis of multiple cell based in vitro assay screening efforts.
• Develops, optimizes, validates, and utilizes robotic automation protocols on Agilent Bravo, BioMekFX, and BioMekNX for high throughput tissue culture needs and screening assay processes.
• Assists with the development and application of immunofluorescence assays with GE InCell and Wallac EnVision.

South San Francisco

• Simultaneously supported and collaborated with multiple research groups and projects by supplying and maintaining high quality plasmid DNA library for in vitro and in vivo screening.
• Prepared, amplified, and purified plasmid DNA through mini/maxi/mega-prep of E.coli. bacterial cultures.
• Assisted the Automation Department with the archiving, tracking, and management of cDNA library records.
• Subcloned plasmid DNA into Invitrogen Gateway and RIPPS SM vectors by the use of PCR purification, annealing, ligation, BP, and LR reaction techniques.
• Performed quality control of plasmid DNA by restriction digest, agarose gel electrophoresis, NanoDrop ND100, Innotech Alpha Imager, and gel extraction purification.
• Executed specialty and high throughput plasmid DNA transformations into competent Invitrogen cells via chemical transformation and electroporation.

Inventory of confidential and non-confidential patents and associated documentation.

Preparation of laboratory solutions and equipment for General Chemistry Laboratories.Chemical and supply inventory for organic and inorganic compounds.Hazardous waste and chemical management per EPA, MSDS, and Santa Clara Country requirements.Data Entry and analysis.

Data acquisition, analysis, and entry in partnership with in-house mathematician.Worked closely with representatives from 3rd party installers and in-house IT.

Bs, Combined Sciences

Activities and Societies: Laboratory Teaching Assistant for Paul Gendler in Organic Chemistry and Elizabeth Thomas in General Chemistry..

Education record

Activities and Societies: Laboratory Teaching Assistant for Homer Tong in Chemistry 50. Completed transfer requirements and was accepted.