Gregory Pirrone Email & Phone Number

Leverage automated and high-throughout analytical platforms, including peptide mapping, MALDI-MS, Pro-A Purification, Ab titer, UPSEC, RPLC, CE-SDS and N-glycan analysis, to enable cell line development, clonal selection, biologics developability and characterization in the discovery space.

Solve complex analytical problems at the interface of chemistry, biology, and lab informatics disciplines to enable discovery and development of active pharmaceutical ingredients (APIs) • Developed and validated robust analytical methods for small and large molecule APIs involving spectroscopy, chromatography, high-resolution mass spectrometry and tandem mass spectrometry techniques• Developed and programmed automated MALDI-MS screening platforms for ultra-high throughput analytical screening for bio-catalytic, chemical or biochemical assays• Developed and performed structure elucidation mass spectrometry methods to target site-specific modifications for large molecule platforms, including proteins, protein conjugates and nucleic acids• Performed timely and successful analytical method transfers to multiple partner groups at multiple sites• Developed robust analytical methods according to best practices, routinely employ fast, high-throughput chromatography and chromatographic simulation software (ACD Labs) for enhancing efficiency and productivity• Strong written and oral skills, ability to understand and meet project deadlines and schedules

Boston, Ma, Us

Investigated conformation and dynamics of peripherally associated membrane proteins by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) • Investigated protein structure and dynamics at lipid membranes• Optimized expression of post-translationally acylated membrane proteins• Purified proteins using Ni-NTA affinity column chromatography• Maintained proper cell culture conditions competent for protein expression• Characterized recombinant proteins using gel electrophoresis, mass spectrometry and UV-Vis spectroscopy• Optimized analytical strategies for membrane protein analysis using lipid mimetics• Performed comparability experiments between lipid bound and lipid free proteins • Implementing neutron reflectometry with HDX and Langmuir monolayers for structuralcharacterization

Ca, 92121, Us

• Coupled streptavidin and biotin to Luminex beads to be used in Flow Cytometry analysis• Analyzed antigen-antibody binding via indirect ELISA assays• Produced and tested material to be used in single-antigen kits to monitor organ transplant patients• Calibrated and performed routine maintenance on laboratory instruments• Prepared buffers and gels to be used in future procedures

Doctor Of Philosophy - Phd, Chemistry

Bachelor Of Science - Bs, Chemistry