Gregory Severson Email & Phone Number

Kirkland, Wa, Us

Bothell, Washington, United States

Everett, Washington

• Assayed preclinical and clinical samples using ELISA and MDS• Developed, verified and modified GLP assay worksheets • Screened and titrated samples for Anti-Drug antibodies (ADA)• Collaborated as the Analytical Biology represented to the Alta Sciences safety comity

Bellevue, Wa

• Troubleshot ELISA assays, Luminex/Magpix assays and Lateral Flow Assays on plasma samples toward developing a disease severity test for third world clinics

Cloning of protein expression system• Assembled protein expression cassettes using various molecular biology methods, including Restriction enzyme, Gibson/seamless cloning (4 fragments) and Sight-Directed Mutagenesis, for transformation into Fungal cell lines• Cloned Crisper/Cas9 knock-out and donor vectors and gRNA expression vectors• Cloned vectors containing recyclable pyrG (URA3) marker into Trichoderma cells• Transforming fungal cell lines for protein expression systems used in manufacturing • Transcribed guide RNA (gRNA) for Crispr/Cas9 directed gene knock-outs and replacement of Trichoderma genes with proprietary expression proteins• Optimized gene gun and protoplast methods for fungal transformation• Screened fungal clones to determine the best viable clones for protein production • Screened fungal clones on 5FOA to recycle pyrG (URA3) marker for further transformation

Greater Seattle Area

Preformed qPCR, mammalian cell culture, cell based assays, western blots for publication

Greater Seattle Area

Working on Assay development, global test for Hemostasis.

Bothell, Wa. Usa

This position is a molecular biologist position performing in vitro and ex-vivo assays to support Marina's in vivo discovery biology group. Techniques include: mRNA isolation, q-rtPCR, Taqman assay, northern blot, 5' RACE assay, ELISA, tissue culture, SDS Page, western blot, fluorescent microscopy, microbial biology, bacterial transformation, restriction digestion, biochemical assays, in vivo dosing and sample collection, Influenza based viral assays, transfections of in vitro cell culture, TCID50, plaque assay, writing protocols, preparing data for publication, training of new staff, and preparing medias and chemicals for laboratory use.

La Jolla, San Diego, California, United States

Managed laboratory, purchased supplies and large equipment, managed the budget for purchasing items needed to start up and new lab, supervised research animal colony, and managed junior staff and work-study students Imaged tissue with a fluorescent microscope, and prepared images for publicationWorked under BSL II/III conditions during both in vivo, ex vivo and in vitro assaysPerformed a variety of molecular, protein and cellular biology experimentsCreated new stem cell lines from knockout mouse brains for basic developmental biology studies into the effects of cortical steroids on neonate brain development