Christopher Grube, Phd Email & Phone Number

South San Francisco, California, United States

• Led ligand-binding immunoassay validations from study initiation through study closure phases including the authoring of study protocols and study closure reports (e.g., validation reports)
• Co-developed a novel Surface Plasmon Resonance (SPR) coupled method to generate reference standard assignments for anti-capsular polysaccharide antibodies and published findings
• Managed a global technology transfer project by transferring a multiplex immunoassay to four different academic, government, and industry laboratories around the world, functioned as the single point of contact and.
• Served as the clinical assay lead on a cross-functional drug development team by actively participating in global meetings, leading the assay sub-team meeting series, and authoring sub-sections of technical reports and.
• Routinely performed large data set analyses and prepared high-quality visuals and presentations using advanced Microsoft Office (e.g. Excel, PowerPoint) skills and statistical analysis software (e.g. GraphPad)
• Coached and developed a team of four direct reports that were responsible for clinical assay performance and lifecycle monitoring via Quality Control (QC) and proficiency panel data trends across eight vaccine programs

Greater New York City Area

• Performed clinical testing on a variety of sample types (e.g., serum, plasma, and urine) in high-throughput, GCLP regulated laboratories to support Phase I-IV vaccine clinical studies using ligand-binding immunoassays.
• Supported critical reagent qualifications and assay qualifications/validations across multiple assays by executing assay runs, reviewing data, and presenting data to key stakeholders for endorsement
• Participated in weekly compliance audits to ensure labs remained GCLP compliant and audit ready
• Performed end-user acceptance testing for data-analysis software to ensure both technical and end-user requirements were met and collaborated with programmers to resolve issues prior to migrating to production

Orlando, Florida Area

• Developed and published 3 target-based, high-throughput screening (HTS) assays in support of dissertation titled “Multi-target high throughput screening assays for antimicrobial drug discovery”
• Completed small library screens using in-house assays and confirmed primary hits using orthogonal methods
• Contributed to the characterization of 3 novel tRNA-dependent lipid modification pathways
• Cloned and purified 50+ unique protein constructs to support projects using the following methods: Bacterial culture methods, DNA extraction, PCR, gel electrophoresis, molecular cloning, DNA sequencing, protein.
• Performed protein structure-function studies using site-directed mutagenesis, thermal shift assays, circular dichroism, C14-radiolabeled substrates, and TLC for lipid product purification and MS analysis
• Taught ~300 undergraduate students molecular biology techniques and chromatography methods for target molecule purifications (e.g., SEC, IEX, HPLC) in a hands-on laboratory methods course across multiple semesters

Lake Nona, Florida

• Implemented molecular biology methods (e.g., cloning, qPCR, gel electrophoresis, and sequencing) to map the 5’-ends of Borrelia burgdorferi transcriptome during mammalian infection
• Performed daily lab technician responsibilities including media preparation, ordering assistance, general lab maintenance and compliance

Doctor Of Philosophy - Phd, Biomedical Sciences, General

Activities and Societies: Biomedical Sciences Graduate Student Association (BSGSA)

Bachelor'S Degree, Biomedical Sciences, General

Activities and Societies: American Society for Microbiology (ASM), Golden Knights Bowling