Manage day to day operation of Transporter and Permeability group in Drug Metabolism and Pharmacokinetics (DMPK) departmentDesgin and lead in vitro permeability, uptake, and inhibtion studies of transporters (including Caco-2, MDCK-MDR1, PAMPA, efflux and uptake transporters like OAT1, OAT3, OCT1, OCT2, OATP1B1, OATP1B3, OATP2B1)Supervise junior scientists, conduct process improvement and optimization, ensure personnel's compliance of compnany's regulations Prepare standard… Show more Manage day to day operation of Transporter and Permeability group in Drug Metabolism and Pharmacokinetics (DMPK) departmentDesgin and lead in vitro permeability, uptake, and inhibtion studies of transporters (including Caco-2, MDCK-MDR1, PAMPA, efflux and uptake transporters like OAT1, OAT3, OCT1, OCT2, OATP1B1, OATP1B3, OATP2B1)Supervise junior scientists, conduct process improvement and optimization, ensure personnel's compliance of compnany's regulations Prepare standard operation procedure (SOP), perform cost analysis and prepare quotation for in vitro Adsorption, Distribution, Metabolism, and Excetion (ADME) studies Show less

Made a total knockout mice to study function of tight junction trans-membrane protein Claudin-18, studied transport and barrier properties of Claudin-18 knockout mice: cultured mouse alveolar epithelial cell monolayer and measured its permeability to different-sized solutes and trans-epithelial resistance; measured in vivo permeability of alveoli to fluorescently-labeled particles and proteins and alveolar fluid clearance; investigated expression and localization of other tight junction… Show more Made a total knockout mice to study function of tight junction trans-membrane protein Claudin-18, studied transport and barrier properties of Claudin-18 knockout mice: cultured mouse alveolar epithelial cell monolayer and measured its permeability to different-sized solutes and trans-epithelial resistance; measured in vivo permeability of alveoli to fluorescently-labeled particles and proteins and alveolar fluid clearance; investigated expression and localization of other tight junction proteins like ZO-1, ZO-2, occludin and their interaction with Claudin-18Studied proliferation, apoptosis, and tumor formation in Type II alveolar epithelial cells in Claudin-18 knockout mice, checked their organ sizes and tumor formation, investigated signaling pathways for regulation of organ size and tumoregenesis; developed novel drugs to target and inhibit growth of lung tumor Show less

Department of Biomedical EngineeringSchool of Egineering

Developed in vitro Blood-Brain Barrier(BBB) models for studying drug transport to the brain by coculturing mouse endothelial cells and rat astrocytes, measured hydraulic conductivity, solute permeability, and transendothelial electric resistance(TEER) of the in vitro BBB model, investigated protein and gene expression of endothelial cells, and studied the interaction between endothelial cells and extracellular matrix Measured permeability of rat brain microvessels to charged proteins… Show more Developed in vitro Blood-Brain Barrier(BBB) models for studying drug transport to the brain by coculturing mouse endothelial cells and rat astrocytes, measured hydraulic conductivity, solute permeability, and transendothelial electric resistance(TEER) of the in vitro BBB model, investigated protein and gene expression of endothelial cells, and studied the interaction between endothelial cells and extracellular matrix Measured permeability of rat brain microvessels to charged proteins using a quantitative fluorescence video microscopy to study the effect of molecular charge on the transport of solutes across the BBB Developed mathematical models to predict permeability of brain microvessels to water and hydrophilic solutes through paracellular pathway for development of effective drug delivery method to the central nervous system Developed a mathematical model to investigate the transport mechanism of cationic nanoparticles across the BBB through adsorptive endocytosis, predicted optimal size range for the nanoparticles to cross the BBB and its permeability Show less

Studied biophysical responses and transport parameters of reproductive cells and tissues at different temperatures and in different mediums to optimize the cryopreservation procedure