SKILLS• Molecular cloning: design and generate DNA constructs, DNA isolation, PCR, site-directed mutagenesis, preparation and transformation of competent cells, maintenance of bacterial strains.• Protein expression: expert in Bacterial expression, and limited training in Baculovirus expression.• Protein purification (both soluble and inclusion body) with metal-affinity, ion-exchange, hydrophobic interaction, size exclusion chromatography using gravity column, FPLC, and HPLC.• Cell-based assays: calcium flux assay, migration assay, membrane protein expression analysis by flow cytometry, receptor conformational change analysis by FRET; Generic CRISPR-based genome-editing technic. • Protein analytical methods, including enzymatic activity assay by Stopped-flow fluorescence; protein-protein/metal ion interaction monitored by two-dimensional NMR; protein folding and stability analysis by Circular Dichroism (CD); protein molecular weight determination by MALDI-TOF Mass Spectrometry; protein chemical cross-linking and characterization by SDS-PAGE; protein detection by western blot.• Protein structure determination and protein dynamics analysis by multi-dimensional Nuclear Magnetic Resonance (NMR) techniques.• Familiar with common structure visualization softwares (Pymol, MolMol, RasMol) and basic bioinformatics databases (NCBI, BLAST, GenBank, SDSC Biology WorkBench).• Independent in experimental design and problem solving.• Excellent communication and teamwork skills.• Fluent in English, Mandarin, and Cantonese.