Hailey Cambra Email & Phone Number

Boston, Massachusetts, United States

I am currently working under Yi-Li's leadership in Tudor Fulga's group within the Duchenne Muscular Dystrophy team. I am leveraging the skills gained from my training experience to progress the gene editing and cell therapy technologies we are working on as a team.

Boston, Massachusetts, United States

• Human Disease Modeling and Mechanisms, Boston Research, Sickle Cell Disease small molecule program (Manager: Caline Matar)Development of physiologically relevant erythroid cell lines to interrogate the biology of the.
• Developed several immortalized erythroid cell lines with endogenous mutants of our target of interest with CRISPR/Cas9 genome editing.
• Assessed phenotypic outcomes of mutagenized lines using flow cytometry assays, a nano luciferase-based assay, and western blot.Automation and execution of a saturation mutagenesis screen of our target of interest in.
• Designed and carried out optimization experiments to test all conditions of the reverse transfection-based assay in 384 well plates.
• Designed, developed, and executed automation protocol for saturation mutagenesis screen.
• Developed a computational protocol (python) to assess quality of the screen and analyze results.

Boston, Massachusetts, United States

• Investigation of the origin and fate of Clu+ stretch-associated cells in intestinal organoids (2021-2023: with Allon Klein and Ophir Klein, unpublished)
• Further optimized sandwich culture protocol from Cambra et al., 2022.
• Completed irradiation assay optimization for murine intestinal organoids in sandwich cultures.
• Completed long-term live imaging assays pre- and post-irradiation alongside healthy control organoids to assess: regeneration, apoptosis, and Clu+ cell signature association with either process post-injury.
• Assessed proliferation association with Clu+ cell signature via EdU assay.Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids (2020-2021: with Allon Klein and Ophir Klein; featured in.
• Characterized the role of inflation and mechanosensitive ion channels in shaping the stem cell niche ex vivo in collaboration with Naren Tallapragada.

Wellesley, Massachusetts, United States

I mentored two undergraduates full-time for 2 weeks to teach them skills in laboratory techniques, research, and science communication.

Worcester, Massachusetts, United States

• I worked for several years under the guidance of Pamela Weathers studying the metabolic pathway producing the anti-malarial drug artemisinin in the plant Artemisia annua. Projects from my time in Pamela Weathers' lab.
• Spectrophotometric Aluminum Chloride assay of Artemisia annua extracts for Flavonoid content to analyze harvesting practices.
• TLC Fingerprinting of high artemisinin producing and glandless Artemisia annua
• High throughput aluminum chloride assay method development

Worcester, MA

I served a 15 minute lecture and headed an hours long laboratory course for undergraduates in general chemistry from my second to final years as a student at Worcester Polytechnic Institute in this position.

Los Alamos, New Mexico, United States

As part of the Department of Energy's Science Undergraduate Laboratory Internship Program (SULI), I worked in the lab of Dr. David Fox in the B-11 Division under the mentorship of Lucas Harrington for one summer. I learned molecular biology techniques in a project where the ultimate goal was reconstruction of a specialized metabolic pathway from high lipid.

Fall River, MA

I worked with Dr. Chen-Lu Yang in my first research experience when I was a senior in high school, and returned the following summer after my first year of college. I learned basic aseptic technique, the processes included in biofuel production from microalgae, and fostered a love for benchwork and scientific research from this experience. I spent one.

Education record

Activities and Societies: Systems Biology DEI Student Working Group (2020-2022)

Bachelor Of Science - Bs, Biochemistry, Cumulative: 3.8/4.0, Major: 3.9/4.0